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Merck
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主要文件

TMS-012

Sigma-Aldrich

Dulbecco's Phosphate Buffered Saline

1X, liquid, w/o Ca++ & Mg++, Endotoxin-Free

别名:

DPBS, PBS, Phosphate buffered saline

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About This Item

分類程式碼代碼:
12352207
eCl@ss:
32160405
NACRES:
NA.75
价格与库存信息目前不能提供

产品名称

不含内毒素Dulbecco′s PBS (1X) (w/o Ca++ & Mg++), Cell Culture

品質等級

形狀

liquid

製造商/商標名

Chemicon®

技術

cell culture | mammalian: suitable

一般說明

无内毒素Dulbecco’s PBS(1X)(不含Ca++&Mg++)通过0.1微米过滤器无菌过滤,认为无内毒素(<0.005 EU/mL)。

應用

无内毒素Dulbecco PBS (1X)(无Ca++ & Mg++)用作以下过程中的洗涤缓冲液:
  • 结肠固有层细胞的分离
  • 通过诱导多能干细胞(iPSC)形成畸胎瘤
  • 从散装支架生产弹性多孔可注射(EPI)生物材料

品質

外观:澄清液体/无颗粒
无菌测试:生长为阴性
pH:7.0-7.2
渗透压:260-305 mOsm/kg
内毒素:<0.005 EU/mL

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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实验方案

Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Questions

  1. Is this made with Bacteriostatic water? I need to reconstitute an enzyme with endotoxin free PBS but the enzyme is inhibited by bacteriostatic water.

    1 answer
    1. The method of preparation is considered proprietary. However, the term 'bacteriostatic' typically implies the presence of benzyl alcohol. No benzyl alcohol is present in the water used to prepare this product.
      The composition is as follows:
      0.2 g/L KCL
      0.2 g/L KH2PO4
      8.0 g/L NaCl
      1.15 g/L Na2HPO4 (anhydrous)

      With the pH adjusted to pH 7.0 to 7.1 using HCl.

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