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Merck
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主要文件

SLM-241

Sigma-Aldrich

DMEM - high glucose

Complete medium, with ʟ-glutamine and sodium pyruvate and 10% FBS, liquid, sterile-filtered, suitable for cell culture

别名:

DME, DMEM with 10% FBS, Dulbecco′s Modified Eagle′s Medium - high glucose

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About This Item

UNSPSC代码:
12352207
eCl@ss:
32160801
NACRES:
NA.75
价格与库存信息目前不能提供

产品名称

DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS, contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS

质量水平

300

表单

liquid

技术

cell culture | mammalian: suitable

相关类别

一般描述

Dulbecco′s Modified Eagle (DMEM) Complete Medium contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS. DMEM Complete Medium is a convenient sterile solution for the culture of primary mammalian cells including mouse and chicken cells, and a variety of normal and transformed cells.

应用

DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS has been used:
  • to culture human gingival fibroblasts[1]
  • to culture mouse fibroblast cells[2]
  • as an overlay medium for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plaque assay[3]

质量

pH= 7.2-7.5
Osmolality= 320-346
Endotoxin= ≤ 2EU/mL
Mycoplasma= Negative
Sterility Tested= Negatvie for Growth
Appearance= Orange/ Red Clear Liquid
Cell Line Tested= HT-22 Human Neuronal Cells

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Natural flora is the richest source of novel therapeutic agents due to their immense chemical diversity and novel biological properties. In this regard, eighteen natural products belonging to different chemical classes were evaluated for their thymidine phosphorylase (TP) inhibitory activity.
Leonard F Blackwell et al.
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The patterns of a woman's normal ovarian activity can take many forms from childhood to menopause. These patterns lie on a continuum ranging from no ovarian activity to a fully fertile ovulatory cycle, but among the other defined patterns are
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Although coronavirus disease 2019 (COVID-19) causes cardiac dysfunction in up to 25% of patients, its pathogenesis remains unclear. Exposure of human induced pluripotent stem cell (iPSC)-derived heart cells to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) revealed productive infection and

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Questions

1–3 of 3 Questions  

  1. What is the composition of this product?

    1 answer

    1. The product is a comprehensive DMEM formulation that includes high glucose with 2mM L-glutamine, sodium pyruvate, and 10% FBS.

      Helpful?

  2. Can you provide the formulation for this media?

    1 answer

    1. The formulation of this media is proprietary. The following is known:

      Glucose: 4.5g/L (high Glucose Formulation)

      If you need to determine the presence or absence of a particular component or its concentration, please contact Technical Service with justification. For example, if the knowledge will support a specific downstream assay, we will then request this information. Please note that we cannot guarantee that this will be provided.

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  3. What is the formulation information available as compared to other DMEMs?

    1 answer

    1. This product is considered a complete DMEM formulation containing high glucose with 2mM L-glutamine, sodium pyruvate, and 10% FBS. Please see the link below to review and compare other classical DMEM formulations: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/380/910/d0572for.pdf

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