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Merck
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主要文件

SCT210

Sigma-Aldrich

BioTracker FerroOrange亚铁橙色活细胞染料

Live Cell imaging probe that specifically detects labile ferrous (Fe2+) ions in live cells.

别名:

senescent cells IHC kit

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.21
价格与库存信息目前不能提供

表单

lyophilized

应用

cell analysis

检测方法

fluorometric

一般描述

铁是人体内最丰富的过渡金属，参与多种重要的生物学过程，包括呼吸、氧输送以及与氧协作产能。铁在活细胞中主要以二价铁(Fe2+)和三价铁(Fe3+)离子形式存在。作为细胞氧化损伤的主要贡献者，Fe2+可与氧、超氧化物和过氧化氢(H2O2)反应制造有害的活性氧，因此与癌症和神经退行性疾病等重病发生有关联。BioTracker FerroOrange亚铁橙活细胞染料是专门用于检测不稳定二价铁离子(Fe2+)的橙色荧光探针。在三价铁离子(Fe3+)或除铁以外的其他二价金属离子存在的情况下，荧光强度不会增加。也不会和铁蛋白或其他物质中的络合铁发生反应。FerroOrange亚铁橙因其高细胞通透性和低细胞毒性而适合活细胞成像。光谱特性：最大吸收波长： 542 nm最大发射波长：572 nm研究类别：活细胞染料

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Questions

1–4 of 4 Questions  

  1. Can this kit be used for suspension cells?

    1 answer

    1. Yes, this product is suitable for use with suspension cells.

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  2. Is it possible to store SCT210 after reconstitution?

    1 answer

    1. The available datasheet for SCT210 provides guidance in the Reagent Preparation section, stating, "Use neutral buffer or culture medium for dilution of the DMSO solution and use immediately upon dilution. The dye could be oxidized in acidic solutions." However, there are no recommendations or data suggesting that the solutions are stable when stored. It's important to note that the package is shipped in single-use vials.

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  3. Is it possible to detect SCT210 FerroOrange by flow cytometry?

    1 answer

    1. The website indicates an excitation wavelength of 542 nm and an emission wavelength of 572 nm for the SCT210 FerroOrange. The product is claimed to be suitable for live cell imaging, which typically implies the use of a confocal microscope. However, given that this is a fluorescent dye, it seems reasonable to consider that the staining could be assessed using a flow cytometer. There is currently no available data documenting its use on a flow cytometer. To use it on a flow cytometer, the cells would need to be in a single cell suspension. Additionally, it is expected that the cells could be visualized using a fluorescent microscope.

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  4. What cells can be used as a positive control for SCT210 (BioTracker™ FerroOrange Live Cell Dye)?

    1 answer

    1. The HepG2 cell line is recommended as a positive control for SCT210 (BioTracker™ FerroOrange Live Cell Dye).

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