SCM013
NDiff Neuro-27培养基补充剂(100x)
NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants & other factors that has been developed for the in vitro propagation & maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium.
别名:
NDiff培养基添加剂, Neuro-27培养基添加剂
登录查看公司和协议定价
所有图片(1)
About This Item
UNSPSC代码:
12352207
eCl@ss:
32160801
NACRES:
NA.75
价格与库存信息目前不能提供
推荐产品
质量水平
表单
liquid
制造商/商品名称
Chemicon®
Millipore
技术
cell culture | stem cell: suitable
输入
sample type: mouse embryonic stem cell(s)
运输
dry ice
一般描述
NDiff Neuro-27培养基补充剂是一种无血清补充剂,含有抗氧化剂和其他因子,专门开发用于在无血清培养基中体外繁殖和维持未分化的鼠胚胎干(ES)细胞(Nichols J,2006)。该产品还可用于将鼠ES细胞分化为有丝分裂后神经元,特别是通过单层分化(Ying QL,2003)。
外形
Ndiff Neuro-27培养基补充剂以水溶液形式提供。必须针对特定用途和细胞类型确定培养基补充剂的最佳工作浓度。在大多数情况下,1:40-1:200的稀释度是合适的。 通常,将Neuro-27稀释为1:100,以进行小鼠ES细胞的自我更新和神经分化。
储存及稳定性
在-20°C下保存直至有效期。避光保存。解冻后,将未使用的部分等分,并储存在-20°C下直至将来使用。一旦添加到细胞培养基中,产品在4°C下可稳定保存四周。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Defined conditions for neural commitment and differentiation
Ying, Qi-Long and Smith, Austin G
Test, 365, 327-341 (2003)
Qi-Long Ying et al.
Nature biotechnology, 21(2), 183-186 (2003-01-14)
Mouse embryonic stem (ES) cells are competent for production of all fetal and adult cell types. However, the utility of ES cells as a developmental model or as a source of defined cell populations for pharmaceutical screening or transplantation is
Jennifer Nichols et al.
Methods in molecular biology (Clifton, N.J.), 329, 91-98 (2006-07-19)
The availability of murine embryonic stem (ES) cells has revolutionized the study of mammalian development and disease. We recently developed a culture medium that has enabled us to identify the essential signaling pathways required for maintenance of pluripotency in vitro.
商品
Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.
实验方案
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
Active Filters
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门