SCC297

N2A Psen1/Psen2 Double Knockout Mouse Neuroblastoma Cell Line

Mouse

• UNSPSC代码: 41106514
• NACRES: NA.81

属性

• 产品名称: N2A Psen1/Psen2 Double Knockout Mouse Neuroblastoma Cell Line,
• 生物来源: mouse
• 包装: vial of >1X10⁶ cells
• 制造商/商品名称: Millipore
• 生长模式: N/A
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

一般描述

Early-onset Alzheimer s disease (AD), also termed autosomal dominant AD with early onset (ADAD), is a devastating neurodegenerative condition that is strongly linked to familial inheritance. The main cause of early-onset/familial AD is autosomal dominant

应用

• Each vial contains >1X10⁶ viable cells.

Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells are verified to be of mouse origin and negative for inter-species contamination from rat, Chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
Cells are negative for mycoplasma contamination.

The N2A Psen1/Psen2 cell line is a model for studies of presenilin function in the pathology of familial Alzheimer′s Disease. Early-onset Alzheimer′s disease (AD), also termed autosomal dominant AD with early onset (ADAD), is a devastating neurodegenerative disease that is strongly linked to familial inheritance. The main cause of early-onset/familial AD is autosomal dominant mutations in APP (amyloid precursor protein), PSEN1 (presenilin-1) or PSEN2 (presenilin-2).1 As catalytic subunits of γ-secretase complex, which cleaves APP to form amyloid beta peptides, Psen1 and Psen2 play significant roles in the formation of amyloid plaques. Variations in PSEN1/2 are frequently present in familial early-onset AD cases but their functional significance is unknown. Unraveling the functions of presenilin proteins is thus key to understanding the molecular pathology of AD.

Source
The N2A Psen1/2 cell line was derived via CRISPR/Cas9-editing of the parental N2A mouse neuroblastoma cell line.2 The N2A (Neuro2a) parental cell line was derived from neuroblastoma stem cells of a male strain A/J mouse.4

References
1. Holtzman DM, Morris JC. Goate AM. Sci Transl Med 2011; 3(77): 77sr1.
2. Pimenova AA, Goate AM. Neurobio Dis 2020; 138:104785.
3. Lee E-S, Jeong S-J, Kim Y-H, Jeon C-J. Acta Histochem Cytochem 2015; 48(6): 205-214.
4. Olmsted JB, Carlson K, Klebe R, Ruddle F, Rosenbaum J. Proc Natl Acad Sci USA 1970; 65(1): 129-136.

特点和优势

The N2A Psen1/2 cell line is a unique tool for the assessment of the contribution of PSEN mutations to AD pathology and widens the path to targeted therapies for AD and related neurodegenerative diseases.

储存及稳定性

Cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

其他说明

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable