Unfortunately, the original publication does not specify the sex of the mouse from which the microglia were derived. This information is not available.
价格与库存信息目前不能提供
推荐产品
产品名称
IMG Mouse Microglial Cell Line, IMG microglial cells recapitulate key features of microglial cell activation and may be used to study neuroinflammatory responses underlying Alzheimer′s disease.
生物来源
mouse
质量水平
技术
cell based assay: suitable
运输
ambient
一般描述
Immortalized MicroGlial Cell Line or IMG is an immortalized microglial cell line derived from adult murine brain infected with the v-raf/v-myc retrovirus. IMG cells express markers that are specific to adult primary microglial cells (F4/80 and CD11b) as assessed by flow cytometry and are responsive to pro- and anti-inflammatory signals (1). They have been shown to phagocytose foreign particles and misfolded proteins such as the amyloid-beta peptide (1). IMG cells are rapidly dividing cells that are typically small and round with few or no processes when cultured in medium containing serum.
Reference:
1. McCarthy RC, Lu DY, Alkhateeb A, Gardeck AM, Lee CH and Wessling-Resnick M (2016). Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta. J. Neuroinflammation 13: 21
Reference:
1. McCarthy RC, Lu DY, Alkhateeb A, Gardeck AM, Lee CH and Wessling-Resnick M (2016). Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta. J. Neuroinflammation 13: 21
Microglia are the primary immune cells of the central nervous system (CNS), and account for 10-15% of all cells found within the brain. Microglial cells play an important role in maintaining the health of the CNS by the removal of pathogens, infectious agents and damaged cells through phagocytosis. Microglial cells have also been extensively studied for their harmful roles in a variety of neurodegenerative disease including Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
细胞系描述
Macrophage Cells
应用
Research Category
Immune Response
Inflammation & Immunology
Neuroscience
Immune Response
Inflammation & Immunology
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neuroinflammation & Pain
Neurodegenerative Diseases
Neuroinflammation & Pain
This product is sold solely for research use per the terms of the “Restricted Use Agreement” which govern its use as detailed in the product Data Sheet or Certificate of Analysis. For information regarding any other use, please contact [email protected].
质量
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
储存及稳定性
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Are these microglia from male or female mice?
1 answer-
Helpful?
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What kind of media would be best to use for this cell line?
1 answer-
The recommended medium for this cell line is as follows:
High Glucose DMEM (Cat. No. D6546)
10 % FBS (Cat. No. ES-009-B)
1X L-Glutamine (Cat. No. TMS-002-C)
1X Penicillin-Streptomycin Solution (Cat. No. TMSAB2-C) (optional)Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/145/570/20267563.pdfHelpful?
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