价格与库存信息目前不能提供
推荐产品
用途
sufficient for 100 extractions
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
技術
DNA extraction: suitable
RNA extraction: suitable
nuclear extraction: suitable
protein extraction: suitable
輸入
sample type cells
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
注意：1个测试=处理5 x 107细胞所需的足够试剂。
该试剂盒提供了独特的试剂组合，可用于从细胞质中分离出高度富集的线粒体级分。富集的级分可用于通过蛋白质印迹、ELISA或其他测定法研究目标因子。
成分
线粒体提取缓冲液、细胞液提取缓冲液、蛋白酶抑制剂混合物、还原试剂和用户操作步骤。
警告
毒性：多个毒性值，请参阅MSDS（O）
原則
ProteoExtract 细胞液/线粒体分级分离试剂盒设计用于哺乳动物细胞，以提取丰富的线粒体和胞质级分，从而用于下游分析，如蛋白质印迹。
準備報告
• 蛋白酶抑制剂混合物: 通过添加250 µlDMSO重溶冻干的蛋白酶抑制剂混合物；使用前要充分混合。分装成小份并储存在-20°C。•1X细胞液提取缓冲液混合物：通过用ddH2O以1：4的比例稀释细胞液提取缓冲液制备1X细胞液提取缓冲液。仅准备测定所需的量（5 x 107 细胞需要1 ml 1X 细胞液提取缓冲液混合物）。在使用前添加2 µl蛋白酶抑制剂混合物和1 µl DTT至1 ml 1X 细胞液提取缓冲液中。•线粒体提取缓冲液混合物：仅准备测定所需的量（5 x 107 细胞需要100 µl 线粒体提取缓冲液混合物）。在使用前添加2 µl蛋白酶抑制剂混合物和1 µl DTT至1 ml线粒体提取缓冲液中。
儲存和穩定性
收到货后将试剂盒的全部内容物存放在-20°C。初次使用后，将缓冲液存储在4°C，将DTT和蛋白酶抑制剂混合物存储在-20°C。
其他說明
由于该运输中有害物质的性质，您的订单可能需要支付额外的运输费用。某些尺寸的产品可免除其他有害材料的运输费用。请与您当地的销售办事处联系，以获取有关这些费用的更多信息。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險聲明
危險分類
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
儲存類別代碼
8A - Combustible corrosive hazardous materials
Jing Wang et al.
International journal of oncology, 51(2), 563-572 (2017-06-07)
Acute myeloid leukemia (AML) is the most common acute leukemia in adults. Despite improved remission rates, current treatment regimens for AML are often associated with a very poor prognosis and adverse effects, necessitating more effective and safer agents. B-cell leukemia/lymphoma 2
Jung-Da Fang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(3), 4327-4340 (2018-12-20)
Knockdown of the suppression of tumorigenicity 14-encoding type II transmembrane serine protease matriptase (MTP) in neural stem/progenitor (NS/P) cells impairs cell mobility, response to chemo-attractants, and neurovascular niche interaction. In the present study, we showed by Western blot that a
Hao Lu et al.
BMC veterinary research, 9, 217-217 (2013-10-24)
Locoweeds cause significant livestock poisoning and economic loss all over the world. Animals can develop locoism, a chronic neurological disease, after grazing on locoweeds. Oxytropis kansuensis is a variety of locoweed that contains swainsonine as its main toxic ingredient. The
Dominique J Favreau et al.
Journal of virology, 86(1), 81-93 (2011-10-21)
Human coronaviruses (HCoV) are recognized respiratory pathogens. Some HCoV strains, including HCoV-OC43, can invade the central nervous system, where they infect neurons, with unclear consequences. We have previously reported that HCoV-OC43 infection of human neurons activates the unfolded-protein response and
Qiuyun Luo et al.
Oncology research, 28(4), 331-344 (2020-02-26)
Despite therapeutic advances, the effective treatment for relapsed or refractory diffuse large B-cell lymphoma (DLBCL) remains a major clinical challenge. Evasion of apoptosis through upregulating antiapoptotic B-cell lymphoma-2 (BCL-2) family members and p53 inactivation, and abnormal activation of B-cell receptor
Active Filters
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门
