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Merck
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文件

PC213L

Sigma-Aldrich

抗-GABA兔pAb

lyophilized, Calbiochem®

别名:

抗-伽玛氨基丁酸

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

rabbit

品質等級

抗體表格

serum

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

lyophilized

不包含

preservative

物種活性

rat

製造商/商標名

Calbiochem®

儲存條件

OK to freeze

同型

IgG

運輸包裝

ambient

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

一般說明

兔多克隆抗GABA抗体可识别大鼠丘脑和小脑中的GABA。经验证可用于冰冻切片的免疫组织化学。
兔多克隆抗体以冻干未稀释的血清形式提供。可识别GABA。
可识别大鼠丘脑和小脑中的GABA。

免疫原

GABA
大鼠

應用


冰冻切片(1:12,000-1:15,000,ABC检测或1:3000-1:5000,PAP检测;见备注)

警告

毒性:标准处理(A)

外觀

未稀释的血清。

重構

用100 µl无菌蒸馏H₂O复溶冻干抗体。保存时,用无菌PBS或Tris缓冲液稀释,稀释度不高于1:10。复溶小瓶的全部内容物;在运输和处理过程中,冻干颗粒的部分可能已脱落,可能不在小瓶底部。溶解后,等分并冷冻保存(-20°C)。储备溶液在-20°C下可稳定保存至多6个月。避免溶液反复冻融。

分析報告

阳性对照
大鼠丘脑或小脑

其他說明

Sudhof, T.C.1995.Nature375, 645.
Hokfelt, T., et al. 1987.Experientia Suppl.56, 154.
在大鼠丘脑和小脑中检查了抗体特异性。戊二醛是与该抗体一起使用时固定的必要组分。可以根据需要使用更高浓度的戊二醛(1%-2%)。使用竞争性抑制ELISA评价抗体对GABA的特异性。GABA共轭物对照完全消除了标记,而以下共轭物的1000倍过量:丙氨酸,天冬氨酸,β丙氨酸,谷氨酸,甘氨酸,牛磺酸和酪氨酸不抑制与GABA共轭物对照的结合。在单个系统中,应对抗体进行滴定以获得最佳结果。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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