MABT902

Anti-Connexin 43 Antibody, clone P2C4

clone P2C4, from mouse

• 别名: Gap junction alpha-1 protein, CX43, Gap junction 43 kDa heart protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: P2C4, monoclonal
• 物種活性: human, rat, mouse
• 物種活性（以同源性預測）: canine (based on 100% sequence homology), hamster (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), rabbit (based on 100% sequence homology), guinea pig (based on 100% sequence homology)
• 技術: electron microscopy: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_036699
• UniProt登錄號: P08050
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: bovine ... Gja1(281193) ; dog ... Gja1(403418) ; guinea pig ... Gja1(100379273) ; human ... GJA1(2697) ; mouse ... Gja1(14609) ; rabbit ... Gja1(100008935) ; rat ... Gja1(24392) ; rhesus monkey ... Gja1(714344)

说明

一般說明

Gap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase.

特異性

Clone P2C4 detects Connexin 43 in human heart. It targets an epitope within 23 amino acids from the C-terminal region. It primarily binds to the non-phosphorylated isoform around Ser364/365.

免疫原

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of rat connexin 43.

應用

Anti-Connexin 43 Antibody, clone P2C4, Cat. No. MABT902, is a mouse monoclonal antibody that targets Connexin-43 and is tested for use in Electron Microscopy, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Connexin 43 in rat heart tissue.

Immunoprecipitation Analysis: A representative lot detected Connexin 43 in Immunoprecipitation applications (Solan, J.L., et. al. (2003). J Cell Sci. 116(Pt 11):2203-11).

Electron Microscopy Analysis: A representative lot detected Connexin 43 in Electron Microscopy applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).

Immunocytochemistry Analysis: A representative lot detected Connexin 43 in Immunocytochemistry applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).

Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).

品質

Evaluated by Immunohistochemistry in human heart tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Connexin 43 in human heart tissue.

標靶描述

43.03 kDa calculated.

外觀

Format: Purified

其他說明

Concentration: Please refer to lot specific datasheet.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1