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![Anti-phospho-TDP-43 [pSer409] antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody](/deepweb/assets/sigmaaldrich/product/images/407/478/bcd6d544-d187-42ed-8c1e-98bf709b4da7/640/bcd6d544-d187-42ed-8c1e-98bf709b4da7.jpg)
生物源
rat
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
1D3, monoclonal
物種活性
human
技術
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG2aκ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
phosphorylation (pSer409/pSer410)
基因資訊
human ... TARDBP(23435)
一般說明
TAR DNA-binding protein 43 (TDP-43) belongs to the hnRNP protein family and plays an important role in transcription, pre-mRNA splicing, mRNA stability and mRNA transport. It is involved in splicing of the apolipoprotein A-II and cystic fibrosis transmembrane gene. This protein is highly expressed in the pancreas, placenta, lung, genital tract and spleen. Mutations in TDP-43 have been associated with amyotrophic lateral sclerosis, frontotemporal dementia, Parkinson′s disease and Alzheimer′s disease.
特異性
This antibody recognizes TDP-43 when phosphorylated at Ser409/Ser410.
免疫原
Epitope: Ser409/Ser410
Ovalbumin-conjugated linear peptide corresponding to human TDP-43 phosphorylated at Ser409/Ser410.
應用
Anti-phospho TDP-43 (Ser409/Ser410) Antibody, clone 1D3 is an antibody against phospho TDP-43 (Ser409/Ser410) for use in WB, ELISA, IH.
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in hippocampus from FTLD-U brain tissue. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
品質
Evaluated by Western Blot in human placenta cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected phosphorylated TDP-43 on 10 µg of human placenta cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected phosphorylated TDP-43 on 10 µg of human placenta cell lysate.
標靶描述
50/25 kDa Observed
1) The 25-kDa C-terminal fragment is more prone to phosphorylation at Ser409/Ser410 than full-length TDP-43. (Zhang, Y.J., et al. (2009). PNAS. 106:7607-7612.)
2) 50-52 kDa observed MW . (Buratti, E., et al. (2001). The EMBO Journal 20:1774-1784.)
1) The 25-kDa C-terminal fragment is more prone to phosphorylation at Ser409/Ser410 than full-length TDP-43. (Zhang, Y.J., et al. (2009). PNAS. 106:7607-7612.)
2) 50-52 kDa observed MW . (Buratti, E., et al. (2001). The EMBO Journal 20:1774-1784.)
外觀
Protein G Purified
Format: Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
分析報告
Control
Human placenta cell lysate
Human placenta cell lysate
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10-13 - German Storage Class 10 to 13
Zhi Huang et al.
Nature communications, 14(1), 2747-2747 (2023-05-13)
Resilience to Alzheimer's disease is an uncommon combination of high disease burden without dementia that offers valuable insights into limiting clinical impact. Here we assessed 43 research participants meeting stringent criteria, 11 healthy controls, 12 resilience to Alzheimer's disease and
Patrick J Bosque et al.
PloS one, 8(5), e62301-e62301 (2013-05-25)
The transactive response DNA-binding protein (TDP-43) is a major component of the abnormal intracellular inclusions that occur in two common neurodegenerative diseases of humans: (1) a subtype of frontotemporal lobar degeneration and (2) amyotrophic lateral sclerosis. Genetics, experiments in cultured
Pol Andrés-Benito et al.
Journal of neuropathology and experimental neurology, 78(5), 416-425 (2019-04-03)
Dyneins are major components of microtubules. Dynein assembly is modulated by a heterogeneous group of dynein axonemal assembly factors (DNAAFs). The present study analyzes dynein axonemal assembly factor 1 (DNAAF1) and leucine-rich repeat-containing protein 50 (LRRC50), the corresponding encoded protein
Jacob R Mann et al.
Neuron, 102(2), 321-338 (2019-03-04)
TDP-43 proteinopathy is a pathological hallmark of amyotrophic lateral sclerosis and frontotemporal dementia where cytoplasmic TDP-43 inclusions are observed within degenerating regions of patient postmortem tissue. The mechanism by which TDP-43 aggregates has remained elusive due to technological limitations, which
Akila Weerasekera et al.
NeuroImage. Clinical, 27, 102327-102327 (2020-07-13)
Currently TAR DNA binding protein 43 (TDP-43) pathology, underlying Amyotrophic Lateral Sclerosis (ALS), remains poorly understood which hinders both clinical diagnosis and drug discovery efforts. To better comprehend the disease pathophysiology, positron emission tomography (PET) and multi-parametric magnetic resonance imaging
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