MAB80138 anti hMPV antibody was used in Immunofluorescence in the following study. A549 or WI-38 cells were fixed in PBS containing 4% paraformaldehyde for 10 minutes on ice, followed by permeabilization with 0.1% saponin for 30 minutes and blocking with PBS containing 10% fetal bovine serum and 2.5% bovine serum albumin for 60 minutes at room temperature. The primary antibody against hMPV nucleoprotein (N protein) was incubated overnight at 4°C, followed by secondary antibody GAM 647 for 30 minutes at room temperature. Nuclear staining was achieved with DAPI staining for 10 minutes. The study can be accessed at https://pmc.ncbi.nlm.nih.gov/articles/PMC5772477/.
This product is usually provided at a concentration of 1 mg/mL, and the lot-specific Certificates of Analysis can be found on the website: https://www.sigmaaldrich.com/product/Anti-Human-Metapneumovirus-Antibody-clone-507,MM_NF-MAB80138#anchor_COA
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生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
507, monoclonal
种属反应性
human
制造商/商品名称
Chemicon®
技术
ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
western blot: suitable
同位素/亚型
IgG2a
运输
wet ice
一般描述
人类偏肺病毒（hMPV）是副粘病毒亚科肺炎病毒属的一员，2001年首次在荷兰儿科呼吸道标本中检出。它是一种有包膜的多形性病毒，基因组为单股负链RNA。目前已检出两种主要血清型：A 型和 B 型。几项研究在世界各地的标本中发现了hMPV，据估计，到5岁时，70% 的儿童将产生hMPV抗体。
特异性
可识别核蛋白。与所有hMPV亚型（A1、A2、B1、B2）均有反应。
应用
使用该抗人类偏肺病毒抗体（克隆号507）检测人类偏肺病毒，经验证可用于ELISA、FC、IF和WB。
外形
形式：纯化
其他说明
浓度：请参考批次特异性浓缩物的检验报告。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Estimates of individuals exposed to human metapneumovirus in a community-based Taiwanese population in 1999.
Chung-Guei Huang,Kuo-Chien Tsao,Tzou-Yien Lin,Yhu-Chering Huang,Li-Ang Lee,Tzu-Hou Chen et al.
Archives of Virology null
Simon Loevenich et al.
Mediators of inflammation, 2019, 4964239-4964239 (2019-11-07)
Human metapneumovirus (HMPV) may cause severe respiratory disease. The early innate immune response to viruses like HMPV is characterized by induction of antiviral interferons (IFNs) and proinflammatory immune mediators that are essential in shaping adaptive immune responses. Although innate immune
Xiaoyan Li et al.
Intervirology, 58(6), 403-412 (2016-04-21)
No licensed vaccines or therapeutic agents for human metapneumovirus (hMPV) infection exist to date. We aimed to construct a multi-epitope peptide (MEP) of hMPV to show promising results for epitope-based vaccine development. Six independent algorithms were screened to predict B-cell
Youxian Li et al.
Scientific reports, 8(1), 883-883 (2018-01-19)
Thymic stromal lymphopoietin (TSLP) is associated with several allergic diseases including asthma. Two isoforms of TSLP exist in humans, a long form (lfTSLP) and a short form (sfTSLP), displaying distinct immunological functions. Recently, TSLP was found to be upregulated in
Mijia Lu et al.
Nature microbiology, 5(4), 584-598 (2020-02-06)
Internal N6-methyladenosine (m6A) modification is one of the most common and abundant modifications of RNA. However, the biological roles of viral RNA m6A remain elusive. Here, using human metapneumovirus (HMPV) as a model, we demonstrate that m6A serves as a
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Regarding MAB80138, anti hMPV antibody, is there any paper that was published using this antibody for Immunofluorescence? In addition was is the approximate concentration of the antibody that you will send?
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