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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
113-5B7, monoclonal
物種活性
rat, human, bovine, mouse
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同型
IgG3κ
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... MMP14(4323)
特異性
Reacts with human MMP-14, also known as MT1-MMP, with a very slight cross-reactivity to hMMP-3. For unknown reasons this antibody does not react on westerns to CC1043.
免疫原
CDGNFDTVAMLRGEM in the hemopexin-like domain of human MT1-MMP
Epitope: a.a. 319-333
應用
Anti-MMP-14 Antibody, hemopexin domain, clone 113-5B7 is an antibody against MMP-14 for use in ELISA, IH(P) & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
Western Blot: 10 μg/mL, membrane preparations; MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours as positive control. 60-65 kDa proteins are identified.
When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59 kDa.
Immunohistochemistry: Frozen tissue sections; traditional formalin fixation is problematic. Paraffin-embedded tissue sections with PLP fixation required.
Immunoprecipitation
EIA
Optimal working dilutions must be determined by the end user.
When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59 kDa.
Immunohistochemistry: Frozen tissue sections; traditional formalin fixation is problematic. Paraffin-embedded tissue sections with PLP fixation required.
Immunoprecipitation
EIA
Optimal working dilutions must be determined by the end user.
標靶描述
~59-69 kDa
外觀
Protein A purified
Format: Purified
Purified immunoglobulin. Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine Serum albumin.
儲存和穩定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析報告
Control
Breast carcinoma tissue
Breast carcinoma tissue
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Manufactured by Daiichi Fine Chemical Co., Ltd
Manufactured by Daiichi Fine Chemical Co., Ltd
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Dynamic expression of matrix metalloproteinases (MMP-2, -9 and -14) and the tissue inhibitors of MMPs (TIMP-1, -2 and -3) at the implantation site during tubal pregnancy.
Bai, SX; Wang, YL; Qin, L; Xiao, ZJ; Herva, R; Piao, YS
Reproduction (Cambridge, England) null
Jeffrey J Atkinson et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 232(4), 1079-1090 (2005-03-02)
Matrix metalloproteinases (MMPs) are expressed during lung development, but their role may be limited, as mice deficient in MMP-3, 7, 9, or 12 develop a normal adult lung. Because membrane-type 1 matrix metalloproteinase (MT1-MMP) is expressed in the developing lung
Amit Ranjan et al.
BioMed research international, 2014, 804680-804680 (2014-09-03)
Matrix remodeling and invasion of basement membrane are the major determinants of malignant progression. Matrix degrading enzymes play a pivotal role in this process and have been shown to be regulated at multiple levels. Using high metastatic B16F10 and its
Riccardo E Nisato et al.
Cancer research, 65(20), 9377-9387 (2005-10-19)
Matrix metalloproteinase (MMP)-2 and its hemopexin C domain autolytic fragment (also called PEX) have been proposed to be crucial for angiogenesis. Here, we have investigated the dependency of in vitro angiogenesis on MMP-mediated extracellular proteolysis and integrin alpha(v)beta3-mediated cell adhesion
Loss of dipeptidyl peptidase IV immunostaining discriminates malignant melanomas from deep penetrating nevi.
Roesch, A; Wittschier, S; Becker, B; Landthaler, M; Vogt, T
Modern Pathology null
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