ABC12
Anti-Bak (NT) Antibody
from rabbit, purified by affinity chromatography
别名:
Apoptosis regulator BAK, BCL2-antagonist/killer 1, BCL2-like 7 protein, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, pro-apoptotic protein BAK
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所有图片(4)
About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
mouse, rat, human
技術
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... BAK1(578)
一般說明
Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.
特異性
This antibody recognizes BAK.
免疫原
KLH-conjugated linear peptide corresponding to human BAK at and around the N-terminus.
應用
Detect Bak (N-terminus) using this Anti-Bak (N-terminus) Antibody validated for use in WB, IH, IC & IP.
Western Blot (SNAP ID) Analysis: 1 - 4 µg/ml from a previous lot detected Bak on 10 µg of Hek293 cell lysate.
Immunoprecipitation Analysis: For unpurified antibodies. 10 µg from a previous lot immunoprecipitated Bak from 500 µg of NIH3T3 lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Bak in kidney tissue.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Bak in HeLa, NIH/3T3, and A431 cells.
Immunoprecipitation Analysis: For unpurified antibodies. 10 µg from a previous lot immunoprecipitated Bak from 500 µg of NIH3T3 lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Bak in kidney tissue.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Bak in HeLa, NIH/3T3, and A431 cells.
品質
Evaluated by Western Blot in Hek293 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Bak on 10 µg of Hek293 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Bak on 10 µg of Hek293 cell lysate.
標靶描述
~ 24 kDa
聯結
Replaces: 04-433
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Lindsey M Ludwig et al.
Methods in molecular biology (Clifton, N.J.), 1877, 77-91 (2018-12-12)
The BCL-2 family of proteins orchestrates a complex signaling network that governs the balance between cellular survival and death. A comprehensive understanding of the mechanistic interactions between these proteins continues to evolve in normal and malignant cells. The functional variation
Yonghua Zhuang et al.
Journal of virology, 90(17), 7684-7691 (2016-06-17)
The tumor suppressor p53 plays a critical part in determining cell fate both as a regulator of the transcription of several proapoptotic genes and through its binding interactions with Bcl-2 family proteins at mitochondria. We now demonstrate that p53 protein
Barbara Pernaute et al.
Developmental cell, 57(11), 1316-1330 (2022-05-22)
The changes that drive differentiation facilitate the emergence of abnormal cells that need to be removed before they contribute to further development or the germline. Consequently, in mice in the lead-up to gastrulation, ∼35% of embryonic cells are eliminated. This
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