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生物源
mouse
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
PML-97, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~90 kDa
物種活性
hamster, human, mouse
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using 293T cells expressing recombinant PML3
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... PML(5371)
一般說明
Monoclonal Anti-PML (mouse IgG1 isotype) is derived from the PML-97 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant full-length human PML (promyelocytic leukemia) protein. PML is characterized with cysteine-rich zinc-binding domains (RING), two B-boxes, an adjacent leucine coiled coil forming the RBCC or tripartite motif (TRIM), and a nuclear localization signal (NLS). The RING and B-box are important for the interaction of the PML protein with different proteins. In normal cells, the PML protein is found in nuclear bodies (PML NBs) whereas in acute promyelocytic leukemia (APL) patients, the PML NBs are disrupted and delocalized from PML NBs to nucleus and cytoplasm.
The PML gene is located on the human chromosome at 15q24.1.
特異性
Monoclonal Anti-PML recognizes human, mouse, and hamster PML (about 90 kDa)
免疫原
recombinant full-length human PML (promyelocytic leukemia) protein.
應用
Anti-PML antibody, Mouse monoclonal may be used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- indirect immunofluorescence
生化／生理作用
A monoclonal antibody to promyelocytic leukemia (PML) is an important tool for studying PML function in normal and cancerous cells. In normal cells, the PML protein and the PML nuclear bodies play a role in different physiological processes such as: growth control, transcription, DNA repair, DNA replication and RNA transport.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
儲存和穩定性
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Pondering the promyelocytic leukemia protein (PML) puzzle: possible functions for PML nuclear bodies
Borden KLB
Molecular and Cellular Biology, 22(15), 5259-5269 (2002)
Jason Koshy et al.
Cancer genetics, 205(10), 537-540 (2012-09-18)
Acute promyelocytic leukemia (APL) is a well-defined subtype of acute myeloid leukemia (AML) specifically characterized by the t(15;17)(q22;q12) translocation. The t(15;17) results in the fusion of the promyelocytic leukemia (PML) and retinoic acid receptor alpha (RARA) genes. Rare cryptic fusions
Wei Zhou et al.
Cell & bioscience, 10(1), 146-146 (2020-12-24)
Increased reactive oxygen species (ROS) production by arsenic treatment in solid tumors showed to be effective to sensitize cancer cells to chemotherapies. Arsenic nano compounds are known to increase the ROS production in solid tumors. In this study we developed
I-Ting Chen et al.
EMBO reports, 22(12), e52254-e52254 (2021-10-12)
Promyelocytic leukemia protein (PML) is a tumor suppressor possessing multiple modes of action, including induction of apoptosis. We unexpectedly find that PML promotes necroptosis in addition to apoptosis, with Pml-/- macrophages being more resistant to TNF-mediated necroptosis than wild-type counterparts
Joanna Stachecka et al.
Histochemistry and cell biology, 149(2), 113-126 (2017-11-15)
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