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69451-M

Sigma-Aldrich

BL21(DE3)pLysS感受态细胞- Novagen

Escherichia coli, rod shaped

别名:

BL21 cell line, Novagen competent cells

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About This Item

UNSPSC代码:
41106202
NACRES:
NA.81
价格与库存信息目前不能提供

产品名称

BL21(DE3)pLysS感受态细胞- Novagen, BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.

生物来源

Escherichia coli

质量水平

100

制造商/商品名称

Novagen®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

transformation efficiency: >2×107 cfu/μg

运输

dry ice

储存温度

−70°C

一般描述

基因型：F-ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)





本产品含有转基因生物（GMO）。在欧盟范围内，转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击here获得最终用户声明（EUD）表格。
BL21菌株是pET重组体蛋白表达最广泛使用的宿主，其优点是缺乏lon和ompT蛋白酶。

DE3表明宿主是λDE3的溶酶原，因此携带一个由lacUV5启动子控制的T7 RNA聚合酶基因的染色体拷贝。这些菌株适合于通过IPTG诱导从pET载体中克隆的靶基因生产蛋白质。

pLysS菌株表达T7溶菌酶，该溶菌酶在诱导前进一步抑制T7 RNA聚合酶的基本表达，从而稳定编码影响细胞生长和活力的靶蛋白的pET重组体。
BL21宿主菌株，表达T7 RNA聚合酶，也编码T7溶菌酶，在诱导前抑制毒性靶蛋白的基本表达。

组分

0.4 ml1 ml成分

•2 × 0.2 ml5 × 0.2 mlBL21(DE3)pLysS感受态细胞

•2 × 2 ml4 × 2 mlSOC培养基

•2 ng2 ng测试质粒

警告

毒性：多个毒性值，请参阅MSDS（O）

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 2

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Debabrata Das et al.
Science advances, 6(44) (2020-11-01)
Oocyte numbers, a critical determinant of female reproductive fitness, are highly regulated, yet the mechanisms underlying this regulation remain largely undefined. In the Caenorhabditis elegans gonad, RAS/extracellular signal-regulated kinase (ERK) signaling regulates oocyte numbers; mechanisms are unknown. We show that

Questions

1–2 of 2 Questions  

  1. Does chloremphenicol need to be added to the culture media to maintain the presence of the pLys plasmid?

    1 answer

    1. The T7 lysozyme is expressed from a plasmid conferring resistance to chloramphenicol. 69451 will need to be cultured with chloramphenicol to maintain this plasmid.

      Helpful?

  2. What is the difference between the strain expressing pLysE and pLysS?

    1 answer

    1. Products 69451-M and 70236-M are exactly the same competent cells. The only difference is in the packaging. The 70236 is already in 50uL aliquots, which are single-use, each aliquot is used for one reaction. This reference is available in two sizes of 11 or 22 reactions. While product 69451 is available in single 0.4 or 1mL volumes which may be thawed once and dispensed to smaller aliquots.
      The difference between the 69451-M/70236-M products and the CMC0015 BL21 is the type of plasmid that they express for the T7 lysozymes. The expression of T7 lysozyme reduces the basal expression of the target genes, inhibiting the T7 RNA polymerase. Specifically, with the expression of the plasmid pLysE (Ref. CMC0015) a higher expression of the levels of T7 Lysozime is obtained in comparison with the plasmid pLysS (ref. 69451-M/70236-M). Consequently, with reference CMC0015 there is even less risk of basal expression of the target protein in the absence of IPTG stimulation. This could be particularly relevant for toxic proteins that could affect competent cell viability.

      Helpful?

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