product name
BL21(DE3)pLysE化学感受态细胞, for protein expression
生物源
Escherichia coli
等級
for molecular biology
增長模式
adherent or suspension
形態學
rod shaped
技術
microbiological culture: suitable
細胞轉化
competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg
運輸包裝
dry ice
儲存溫度
−70°C
一般說明
The BL21(DE3) pLysE Chemically Competent Cells produce T7 lysozyme to repress low-level transcription from the T7 promoter prior to IPTG induction, helping to stabilize inserts coding for deleterious products.
These cells have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
These cells have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
特點和優勢
- a transformation efficiency of >1 × 107 cfu/μg.
- economical prices
- offering superior value for everyday protein expression work.
成分
- BL21(DE3) pLysE chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
儲存類別代碼
10 - Combustible liquids
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