648310-M

Tris Base，分子生物学级

≥99% (perchloric acid titration), solid, pH 7.0-9.0, solid, Molecular Biology Grade

• 别名: 三（羟甲基）氨基甲烷, 2-氨基-2-(羟甲基)-1,3-丙二醇, THAM, Tris 碱, 氨基丁三醇
• 线性分子式: NH₂C(CH₂OH)₃
• CAS号: 77-86-1
• 分子量: 121.14
• Beilstein: 741883
• MDL號碼: MFCD00004679
• 分類程式碼代碼: 12161705
• NACRES: NA.25

属性

• product name: Tris Base，分子生物学级, TRIS base is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
• 等級: Molecular Biology
• 品質等級: 100
• 化驗: ≥99% (perchloric acid titration)
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 顏色: white
• 有用的pH值範圍: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• 溶解度: water: 500 mg/mL
• 正離子痕跡: heavy metals: ≤0.0005%
• 吸收: ≤0.02 at 280 nm; ≤0.03 at 260 nm
• 異物活動: RNases, DNases, proteases, none detected
• 儲存溫度: 10-30°C
• SMILES 字串: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI 密鑰: LENZDBCJOHFCAS-UHFFFAOYSA-N

说明

一般說明

在 7.0-9.0 的 pH 范围内有效。在 25°C 下的 pK_a 为8.1。

已广泛用作生物缓冲液（吸光度：≤260 nm处0.03；≤280 nm处0.02）。在7.0-9.0的pH范围内有效。25°C时PK_a为8.1。

應用

• A Fast and Automated Melanin-bleaching Method for Histopathologic Evaluation of Pigmented Melanoma Tissues.: This study presents an automated melanin-bleaching technique using a Tris-based buffer to enhance histopathologic evaluation of pigmented melanoma tissues, aiding in more accurate diagnoses (Ugolini et al., 2022).


• Improvement of plant protein solubilization and 2-DE gel resolution through optimization of the concentration of Tris in the solubilization buffer.: Highlights the optimization of Tris concentration in solubilization buffers to enhance protein solubilization and gel electrophoresis resolution, crucial for biochemical studies (Cho et al., 2010).

警告

毒性：刺激剂（B）

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable