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Merck
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主要文件

574795

Sigma-Aldrich

TBE 缓冲液，10X，分子生物学级

A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.

别名:

三-硼酸-EDTA缓冲溶液

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About This Item

UNSPSC代码:
41105319
NACRES:
NA.25
价格与库存信息目前不能提供

等级

Molecular Biology

表单

liquid

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

异质活性

DNase, RNase, protease, none detected

运输

ambient

储存温度

15-25°C

一般描述

10X 浓缩物，可稀释成2X溶液，其中含有89 mM Tris、89 mM醋酸盐和2 mM EDTA，pH~8.3。
三硼酸盐和乙二胺四乙酸（TBE）缓冲液用于DNA和RNA的琼脂糖凝胶电泳。它由一种以中性和阴离子形式存在的弱酸组成（比如，COOH和COO- 物种）。Tris是一种弱碱，以中性或阳离子形式存在（Tris-NH2 和Tris-NH3+）。这些离子保持介质的低导电性，可传输电流以及缓冲pH值。乙二胺四乙酸(EDTA)虽然并非必需，但可用作预防添加，因为它螯合Mg2+离子并使潜在的DNA核酸酶失活。TBE允许DNA片段的有效分辨率，并略微改善较小片段的分离。[1]它还能稳定核酸，防止酶降解。[2]

应用

TBE缓冲液（10倍），分子生物学级 - Calbiochem已用为聚丙烯酰胺测序凝胶的组分，用于通过扩增片段长度多态性（AFLP）进行DNA电泳。[3]

警告

毒性：刺激性（B）

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H360FD

危险分类

Repr. 1B

储存分类代码

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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Marjan Sadat Hosseini et al.
Phytochemistry, 183, 112629-112629 (2021-01-31)
Licorice (Glycyrrhiza glabra L.) is a medicinal plant species valued in many countries in Asia and Europe for its phytochemical characteristics. Licorice biodiversity is becoming threatened nowadays in Iran due to increasing demand and a drastic decline of its natural
Optical properties of buffers and cell culture media for optofluidic and sensing applications
Hoang VT, et al.
Applied Sciences, 9(6), 1145-1145 (2019)
Brian A Sanderson et al.
Analytical biochemistry, 454, 44-52 (2014-03-19)
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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