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537060

Sigma-Aldrich

Propidium Iodide Solution

A convenient form of propidium iodide useful for flow cytometry studies.

别名:

Propidium Iodide Solution

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About This Item

经验公式（希尔记法）:
C27H34I2N4
CAS号:
25535-16-4
分子量:
668.39
MDL编号:
MFCD00011921
UNSPSC代码:
12352200
NACRES:
NA.47
价格与库存信息目前不能提供

质量水平

100

描述

RTECS - SF7949600

表单

liquid

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
protect from light

输入

sample type intact cells

荧光

λex 536 nm
λem 617 nm

运输

ambient

储存温度

2-8°C

SMILES字符串

[I-].I.[N+](CCCN1C2=CC(=N)C=CC2=C3C(=C1c4ccccc4)C=C(C=C3)N)(CC)(CC)C

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

相关类别

一般描述

Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.

生化/生理作用

Cell permeable: no
Primary Target
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no

警告

Toxicity: Standard Handling (A)

外形

50 µg/ml in PBS, pH 7.4.

重悬

Following initial use, aliquot and freeze (-20°C).

其他说明

Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Questions

1–2 of 2 Questions  

  1. what is the concentration of the PI solution

    1 answer

    1. The concentration is 50 µg/ml in PBS, pH 7.4.

      Helpful?

  2. What is the recommended protocol for use/ working concentration?

    1 answer

    1. The working concentration must be determined by the end user.
      In the following reference: Cytometry. 1994 Sep 1;17(1):59-65. PMID: 7528124 DOI: 10.1002/cyto.990170108; 5ug/ML of PI was directly added in the culture medium for 30min. at 37C.

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