推荐产品
product name
佛波醇12-十四酸酯13-乙酸酯, Phorbol-12-myristate-13-acetate, CAS 16561-29-8, is the most common phorbol ester. Activates PKC at nanomolar concentrations.
品質等級
化驗
≥98% (HPLC)
形狀
solid
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
顏色
white
溶解度
DMSO: 10 mg/mL
運輸包裝
ambient
儲存溫度
−20°C
InChI
1S/C36H56O8/c1-7-8-9-10-11-12-13-14-15-16-17-18-29(39)43-32-24(3)35(42)27(30-33(5,6)36(30,32)44-25(4)38)20-26(22-37)21-34(41)28(35)19-23(2)31(34)40/h19-20,24,27-28,30,32,37,41-42H,7-18,21-22H2,1-6H3/t24-,27+,28-,30-,32-,34-,35-,36-/m1/s1
InChI 密鑰
PHEDXBVPIONUQT-RGYGYFBISA-N
一般說明
最常用的佛波醇酯。极强效的小鼠皮肤肿瘤促进剂。即使在nM浓度下也能在体内和体外激活蛋白激酶C。在培养的肝细胞中促进可诱导型NOS的表达。激活Ca2+-ATPase并增强毛喉素诱导的cAMP形成。抑制Fas抗原诱导的凋亡，但在HL-60早幼粒细胞白血病细胞中是诱导凋亡。它的结合是可逆的。还可以提供在DMSO（货号500582）中的10 mM溶液。
极强效的小鼠皮肤肿瘤促进剂。即使在纳摩尔浓度下也能在体内和体外激活蛋白激酶C（PKC）。在培养的肝细胞中促进iNOS的表达。激活Ca2+-ATPase并增强毛喉素诱导的cAMP形成。抑制Fas抗原诱导的凋亡，但在HL-60早幼粒细胞白血病细胞中是诱导凋亡。最常用的佛波醇酯。
生化／生理作用
产物不与ATP竞争。
可逆性：是
细胞渗透性：否
首要靶标
蛋白激酶C
蛋白激酶C
包裝
用惰性气体包装
警告
毒性：有害（C）
準備報告
仅在使用前再用水相缓冲液进行稀释。
重構
溶解后，等分并冷冻保存（-20°C）。无水DMSO储备液可在-20°C最长稳定6个月。DMSO储备液在频繁使用时可能会积水。储备液中存在的水将会加速产物的分解，导致在2-3个月内活性的明显下降。
其他說明
Powell, D.J., et al. 2003.Mol.Cell Biol.23, 7794.
Tepper, C.G., et al. 1995.Proc.Natl.Acad.Sci. USA92, 8443.
Oishi, K., and Yamaguchi, M. 1994.J. Cell.生物化学。55, 168.
Macfarlane, D.E., and O’Donnell, P.S.1993.Leukemia7, 1846.
Ghandi, V.C. and Jones, D.J., 1992.Neuropharmacol.31, 1101.
Hortelano, S., et al. 1992.J. Biol. Chem. 267, 24937.
Kontny, E., et al. 1992.Eur. J. Pharmacol.227, 333.
Zanaboni, P.B., et al. 1992.J. Appl. Physiol.73, 2011.
Saltis, J., et al. 1991.J. Biol. Chem.266, 261.
Beguinot, L., et al. 1985.Proc.Natl.Acad.Sci. USA82, 2774.
Perchellet, J. 1985.Carcinogenesis6, 567.
Nishizuka, Y. 1984.Science255, 1365.
Mastro, A. 1982.Lymphokines6, 263.
Tepper, C.G., et al. 1995.Proc.Natl.Acad.Sci. USA92, 8443.
Oishi, K., and Yamaguchi, M. 1994.J. Cell.生物化学。55, 168.
Macfarlane, D.E., and O’Donnell, P.S.1993.Leukemia7, 1846.
Ghandi, V.C. and Jones, D.J., 1992.Neuropharmacol.31, 1101.
Hortelano, S., et al. 1992.J. Biol. Chem. 267, 24937.
Kontny, E., et al. 1992.Eur. J. Pharmacol.227, 333.
Zanaboni, P.B., et al. 1992.J. Appl. Physiol.73, 2011.
Saltis, J., et al. 1991.J. Biol. Chem.266, 261.
Beguinot, L., et al. 1985.Proc.Natl.Acad.Sci. USA82, 2774.
Perchellet, J. 1985.Carcinogenesis6, 567.
Nishizuka, Y. 1984.Science255, 1365.
Mastro, A. 1982.Lymphokines6, 263.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險分類
Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
儲存類別代碼
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
水污染物質分類（WGK）
WGK 3
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