454578

Anti-Pan-NMDA NR1 Mouse mAb (R1JHL)

lyophilized, clone R1JHL, Calbiochem^®

• UNSPSC代码: 12352203
• NACRES: NA.43

属性

• 生物来源: mouse
• 质量水平: 100
• 抗体形式: culture supernatant
• 抗体产品类型: primary antibodies
• 克隆: R1JHL, monoclonal
• 表单: lyophilized
• 不包含: preservative
• 种属反应性: rat
• 制造商/商品名称: Calbiochem^®
• 储存条件: OK to freeze
• 同位素/亚型: IgG
• 运输: ambient
• 储存温度: 2-8°C
• 靶向翻译后修饰: unmodified

说明

一般描述

Mouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.

Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.

This Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) is validated for use in Immunoblotting for the detection of Pan-NMDA NR1.

免疫原

Rat

a recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1

应用

Immunoblotting (1:300, colorimetric)

Immunoprecipitation (3 µg/200 µg lysate)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from cell culture supernatant.

重悬

Reconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

分析说明

Positive Control
Rat cortex tissue extracts

其他说明

Does not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.

Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci.14, 3180
Ishii, T., et al. 1993. J. Biol. Chem.268, 2836.
Monyer, H., et al. 1992. Science256, 1217.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable