Recombinant, human furin expressed in vaccinia virus (VV:hFUR713t) infected BSC-40 cells. Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like pro-hormone convertase (PC) family. Members of this family cleave pro-hormones, neuropeptide precursors, some viral proteins, and bacterial toxins. The preparation was subjected to UV irradiation for 5 minutes to inactivate any residual virus.
This preparation was subjected to UV irradiation for 5 min to inactivate any residual vaccinia virus.
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of furin that will cleave 1.0 pmol AMC from the substrate pGlu-Arg-Thr-Lys-Arg-AMC per min at 30°C, pH 7.5.
外形
In 20 mM Bis-Tris buffer, 1 mg/ml BSA, pH 7.0.
重悬
Following initial thaw, aliquot and freeze (-70°C).
其他说明
Bassi, D.E., et al. 2001. Mol. Carcinog.31, 224. Bassi, D.E., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10326. Creemers, J.W., et al. 2001. J. Biol. Chem.276, 4211. Wang, X., and Pei, D. 2001. J. Biol. Chem.276, 35953. Molloy, S.S., et al. 1992. J. Biol. Chem.267, 16396.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany