07-307
Anti-phospho-STAT1 (Tyr701) Antibody
Upstate®, from rabbit
别名:
Transcription factor ISGF-3 components p91/p84, signal transducer and activator of transcription 1, signal transducer and activator of transcription 1, 91kD, signal transducer and activator of transcription 1, 91kDa, signal transducer and activator of tr
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About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
推荐产品
生物源
rabbit
品質等級
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
mouse, chicken, human, rat
製造商／商標名
Upstate®
技術
western blot: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
phosphorylation (pTyr701)
基因資訊
human ... STAT1(6772)
一般說明
STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT 1, is activated by a number of different ligands, including IFNalpha, IFNgamma, EGF, PDGF and IL6. Phosphorylation of tyrosine 701 is required for STAT 1 dissociation from IFNGR1, homodimerization, and nuclear translocation. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions.
特異性
Other species cross-reactivity is unknown.
Phosphorylated STAT1
免疫原
Epitope: Tyr701
KLH-conjugated synthetic peptide (C-DDPKRTG[pY]IKTE) corresponding to amino acids 694-705 of mouse STAT1, containing a N-terminal cysteine.
應用
Anti-phospho-STAT1 (Tyr701) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-STAT1 (Tyr701) also known as Transcription factor ISGF-3 components p91/p84 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
品質
Routinely evaluated by immunoblot in RIPA lysates from CNTF-treated SK-N-MC cells.
Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells.
Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells.
標靶描述
92 kDa
外觀
Immunoaffinity Chromatography
Immunoaffinity purified IgG in 0.02 M PBS containing 0.1% Sodium Azide + 50% Glycerol and 5 mg/mL BSA.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
分析報告
Control
Mouse 3T3-L1 adipocytes stimulated with leukemia inhibitory factor (LIF).
Mouse 3T3-L1 adipocytes stimulated with leukemia inhibitory factor (LIF).
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
Hepatitis C virus core protein differently regulates the JAK-STAT signaling pathway under interleukin-6 and interferon-gamma stimuli.
Hosui, Atsushi, et al.
The Journal of Biological Chemistry, 278, 28562-28571 (2003)
Donor T-cell alloreactivity against host thymic epithelium limits T-cell development after bone marrow transplantation.
Hauri-Hohl, MM; Keller, MP; Gill, J; Hafen, K; Pachlatko, E; Boulay, T; Peter et al.
Blood null
Porcine reproductive and respiratory syndrome virus Nsp1? inhibits interferon-activated JAK/STAT signal transduction by inducing karyopherin-?1 degradation.
Wang, Rong, et al.
Journal of virology, 87, 5219-5228 (2013)
Precision-cut slice cultures of tumors from MMTV-neu mice for the study of the ex vivo response to cytokines and cytotoxic drugs.
Nirmala Parajuli, Wolfgang Doppler
In Vitro Cellular & Developmental Biology. Animal null
Induction of type I interferons by a novel porcine reproductive and respiratory syndrome virus isolate.
Nan, Yuchen, et al.
Virology, 432, 261-270 (2012)
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