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生物来源
mouse
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
73P21, monoclonal
种属反应性
human
制造商/商品名称
Upstate®
技术
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... AKT1S1(84335)
一般描述
PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
特异性
Recognizes PRAS40.
This antibody does not cross react with mouse.
免疫原
Recombinant human PRAS40 (full-length) expressed in E. coli.
应用
Anti-Pras40 Antibody, Clone 73P21 is an antibody against Pras40 for use in IP & WB.
Immunoblot Analysis: A 01-1.0 μg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
质量
Routinely evaluated by western blot.
Western Blot Analysis:
A 0.1-1.0 µg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
Western Blot Analysis:
A 0.1-1.0 µg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
目标描述
40 kDa
外形
Affinity purified
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.1% sodium azide.
储存及稳定性
Stable for 1 years at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
RIPA lysates of HeLa cells.
RIPA lysates of HeLa cells.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Evaluating the therapeutic potential of mTOR inhibitors using mouse genetics.
Huawei Li,Jennifer L Cotton,David A Guertin
Methods in Molecular Biology null
David A Guertin et al.
Cancer cell, 15(2), 148-159 (2009-02-03)
mTOR complex 2 (mTORC2) contains the mammalian target of rapamycin (mTOR) kinase and the Rictor regulatory protein and phosphorylates Akt. Whether this function of mTORC2 is critical for cancer progression is unknown. Here, we show that transformed human prostate epithelial
Daniel W D West et al.
The American journal of clinical nutrition, 94(3), 795-803 (2011-07-29)
Ingestion of whey or casein yields divergent patterns of aminoacidemia that influence whole-body and skeletal muscle myofibrillar protein synthesis (MPS) after exercise. Direct comparisons of the effects of contrasting absorption rates exhibited by these proteins are confounded by their differing
Wenting Mi et al.
Oncotarget, 6(16), 13962-13977 (2015-05-12)
The mTORC1 inhibitors, rapamycin and its analogs, are known to show only modest antitumor activity in clinic, but the underlying mechanisms remain largely elusive. Here, we found that activated AKT signaling is associated with rapamycin resistance in breast and colon
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