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Merck
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文件

05-633

Sigma-Aldrich

抗-BrdU抗体,克隆BU-1

culture supernatant, clone BU-1, Upstate®

别名:

Anti-BrdU antibody

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

culture supernatant

抗體產品種類

primary antibodies

無性繁殖

BU-1, monoclonal

物種活性(以同源性預測)

all

製造商/商標名

Upstate®

技術

immunocytochemistry: suitable

同型

IgG2a

運輸包裝

wet ice

目標翻譯後修改

unmodified

一般說明

掺入DNA的溴脱氧尿苷(BrdU)的免疫细胞化学检测是研究正常细胞和赘生性细胞的细胞动力学的有力工具。用胸苷类似物BrdU体外或体内标记肿瘤细胞,然后用特异性抗BrdU单克隆抗体检测掺入的BrdU是定量DNA合成程度的准确而全面的方法。BrdU被掺入S期细胞的新合成DNA中,因此可以提供S期细胞比例的估计值。还可以通过双变量BrdU/DNA流式细胞术分析获得动态增殖信息(例如S期转运速率和潜在的倍增时间)。

特異性

5-溴-2-脱氧尿苷

免疫原

5-碘尿苷:卵清蛋白结合物

應用

用已证明可在ICC中起作用的抗BrdU抗体(克隆BU-1(小鼠单克隆抗体))检测BrdU(也称为溴脱氧尿苷)。

品質

通过免疫细胞化学在HeLa细胞上评价了代表性批次。代表性批次的1:2-1:4稀释液在用95%乙醇,5%冰醋酸固定的HeLa细胞中显示出抗BrdU的阳性免疫染色。使用DNA复制检测试剂盒(目录号17-316)检测该批次。

技术提示:该抗体可识别掺入双链DNA的5-溴-2-脱氧尿苷。不需要DNA变性。

標靶描述

未给出

外觀

4瓶,每瓶含有2.5 mL冻干的小鼠培养物上清液。上清液含有支原体,其贡献促进抗体结合的酶。冻干粉末。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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