推荐产品
生物源
mouse
品質等級
抗體表格
culture supernatant
抗體產品種類
primary antibodies
無性繁殖
BU-1, monoclonal
物種活性(以同源性預測)
all
製造商/商標名
Upstate®
技術
immunocytochemistry: suitable
同型
IgG2a
運輸包裝
wet ice
目標翻譯後修改
unmodified
一般說明
掺入DNA的溴脱氧尿苷(BrdU)的免疫细胞化学检测是研究正常细胞和赘生性细胞的细胞动力学的有力工具。用胸苷类似物BrdU体外或体内标记肿瘤细胞,然后用特异性抗BrdU单克隆抗体检测掺入的BrdU是定量DNA合成程度的准确而全面的方法。BrdU被掺入S期细胞的新合成DNA中,因此可以提供S期细胞比例的估计值。还可以通过双变量BrdU/DNA流式细胞术分析获得动态增殖信息(例如S期转运速率和潜在的倍增时间)。
特異性
5-溴-2-脱氧尿苷
免疫原
5-碘尿苷:卵清蛋白结合物
應用
用已证明可在ICC中起作用的抗BrdU抗体(克隆BU-1(小鼠单克隆抗体))检测BrdU(也称为溴脱氧尿苷)。
品質
通过免疫细胞化学在HeLa细胞上评价了代表性批次。代表性批次的1:2-1:4稀释液在用95%乙醇,5%冰醋酸固定的HeLa细胞中显示出抗BrdU的阳性免疫染色。使用DNA复制检测试剂盒(目录号17-316)检测该批次。
技术提示:该抗体可识别掺入双链DNA的5-溴-2-脱氧尿苷。不需要DNA变性。
技术提示:该抗体可识别掺入双链DNA的5-溴-2-脱氧尿苷。不需要DNA变性。
標靶描述
未给出
外觀
4瓶,每瓶含有2.5 mL冻干的小鼠培养物上清液。上清液含有支原体,其贡献促进抗体结合的酶。冻干粉末。
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our 产品选型工具.
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma.
Testing null
Difference between random and imprinted X inactivation in common voles.
Chromosoma null
Cytometry, 6(6), 506-512 (1985-11-01)
We describe a mouse monoclonal antibody (BU-1) reactive with 5-bromo-2-deoxyuridine (BrdUrd). The antibody is different from previously described BrdUrd monoclonal antibodies in that BU-1 does not require pretreatment of cells with strong DNA denaturants in order for the antibody to
S-phase detection with an antibody to bromodeoxyuridine. Role of DNase pretreatment.
Journal of Immunological Methods, 93, 97-101 (1986)
Journal of molecular cell biology, 11(5), 408-420 (2018-09-15)
DICER1 is a key enzyme responsible for the maturation of microRNAs. Recent evidences suggested that DICER1 and microRNAs expressed in epididymis were involved in the control of male fertility. However, the exact mechanism remains to be elucidated. Here, we created
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门