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Merck

803332

Sigma-Aldrich

磺基-SANPAH(磺基琥珀酰亚胺基6(4′-叠氮基-2′-硝基苯氨基)己酸酯

别名:

(sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino)hexanoate), 1-[[6-[(4-azido-2-nitrophenyl)amino]-1-oxohexyl]oxy]-2,5-dioxo-3-pyrrolidinesulfonic acid monosodium salt, Sulfo-SANPAH

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About This Item

经验公式(希尔记法):
C16H17O9N6NaS
分子量:
492.40
UNSPSC代码:
12352102
PubChem化学物质编号:
NACRES:
NA.22

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表单

powder

质量水平

分子量

492.4

反应适用性

reagent type: cross-linking reagent

储存条件

desiccated

溶解性

water: soluble

官能团

azide

运输

ambient

储存温度

−20°C

SMILES字符串

O=C1CC(S(=O)([O-])=O)C(N1OC(CCCCCNC2=CC=C(N=[N+]=[N-])C=C2[N+]([O-])=O)=O)=O.[Na+]

InChI

1S/C16H18N6O9S.Na/c17-20-19-10-5-6-11(12(8-10)22(26)27)18-7-3-1-2-4-15(24)31-21-14(23)9-13(16(21)25)32(28,29)30;/h5-6,8,13,18H,1-4,7,9H2,(H,28,29,30);/q;+1/p-1

InChI key

XXUXLXCHYVHAOD-UHFFFAOYSA-M

一般描述

ANB-NOS和磺基-SANBAH是异双官能交联剂,含有胺反应性的N-羟基琥珀酰亚胺酯和光活化的硝基苯叠氮化物。NHS酯在pH 7-9缓冲液中与伯氨基(-NH2)有效反应,形成稳定的酰胺键。当暴露在紫外光下时,硝基苯叠氮化合物形成氮烯基团,该基团可以引发双键的加成反应,插入碳氢和氮氢位点,或随后与亲核体(例如伯胺)发生扩环反应。

应用


  • 刚度可调基质上细胞粘附的动态控制,可用于细胞集体迁移的力学生物学分析:本研究在细胞粘附研究中使用Sulfo-SANPAH进行光化学交联(Kamimura et al., 2017)。

  • L929成纤维细胞在ArgGlyAspSer(RGDS)固定化壳聚糖上的附着和增殖评价:本文研究了使用Sulfo-SANPAH增强成纤维细胞在壳聚糖基质上的附着(Karakecili et al., 2016)。

特点和优势

  • 反应基团:磺基氨基磺酸酯和硝基苯叠氮化物
  • 对氨基和任何亲核物质有反应
  • 不可切割
  • n-磺基琥珀酰亚胺基-6-(4′-叠氮基-2′-硝基苯氨基)己酸酯
  • 最佳光解发生在320-350纳米;将辐射对生物分子的损害降至最低
  • 水溶性;不可切割

注意

这种产品对湿气很敏感。小瓶包装在带有干燥剂的可再密封袋中,以减少湿气接触。冷藏后,打开前将小瓶平衡至室温,以减少小瓶内的冷凝。制备新的溶液。不建议储存原液。使用后,将小瓶放回可重新密封的袋子中。封闭袋子,在推荐的温度下储存产品。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

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    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
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      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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