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798088

Sigma-Aldrich

纳米金刚石荧光剂

Nitrogen vacancy ~3 ppm NV centers, 120 nm avg. part. size, 1 mg/mL in deionized water

别名:

纳米金刚石, 金刚石纳米粉末

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5 ML
$502.00

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$502.00

About This Item

线性分子式:
C
UNSPSC代码:
12352302
NACRES:
NA.23

$502.00


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表单

suspension

质量水平

组成

Nitrogen vacancy, ~3 ppm NV centers

浓度

1 mg/mL in deionized water

平均零件尺寸

120 nm

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一般描述

产品可使用羧酸、羟基、酮、醚和/或氢进行功能化。
在荧光纳米金刚石中,金刚石晶格中的氮空位中心提供荧光;与量子点或有机染料不同,这些中心的颜色不会发生光漂白或光致发光。这些突出的性能以及荧光纳米金刚石的生物相容性,大的表面积等。因此它们成为有前途的生物成像探针。
荧光纳米金刚石 (FND) 可以通过将人造金刚石纳米晶置于氦离子下制备。[1]FND 具有生物相容性,其表面易于功能化。它是生物成像中非常有用的工具,主要是因为激光激发时从 FND 的缺陷中心发出的光子很容易穿透组织。FNDs 的发射非常稳定。[1]FND 的平均尺寸是 120 nm。

应用

FND 的潜在应用是作为体内纳米探针用于生物成像、细胞标记和细胞追踪。[1][2]

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Nanodiamonds for optical bioimaging
Y. Y. Hui, C-L Cheng, and H-C Chang
Journal of Physics D: Applied Physics, 43(37), 374021-374021 (2010)
Highly Fluorescent Nanodiamonds Protein-Functionalized for Cell Labeling and Targeting
B-M Chang, H-S Lin, L-J Su, et al.
Advances in Functional Materials, 23(46), 5737-5745 (2013)
Mass production and dynamic imaging of fluorescent nanodiamonds
Chang YR, et al.
Nature Nanotechnology, 3(5), 284-288 (2008)
The Exocytosis of Fluorescent Nanodiamond and Its Use as a Long-Term Cell Tracker
Fang CY, et al.
Small, 7(23), 3363-3370 (2011)

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Fluorescent Nanodiamonds and biofunctionalization strategies are detailed for imaging and bioconjugation applications.

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Questions

1–2 of 2 Questions  
  1. I would like to see the fluorescence response of 798088

    1 answer
    1. Please see the image below to review the spectral data for this product.

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  2. 1. Is your inflorescence nanodiamond dyed?
    2. What are the excitation and emission wavelengths for them? are their quantum yield and brightness information available?
    3. What is the function of the PEG functionalization here? Does it reduce surface tension? Does the functional group affect the fluorescent intensity?
    4. Has it been used for some fluorescent tracing experiments?

    1 answer
    1. 1 The fluorescence originates from the nanodiamonds centers, such as nitrogen-vacancy (NV), NVN, or N3, and not from any fluorescent groups. Therefore, it is not dyed.
      2 The excitation/emission wavelengths are consistent for these products and are based on the nanodiamonds center. The catalog part numbers, center type, excitation peak, emission peak, and brightness information are as follows:
      900173, NVN, 480 nm, 520 nm (green), ~90x vs FITC
      All other products, NV, 570 nm, 680 nm (red), ~70x brighter vs Atto 647 Two important notes provided were:
      Green fluorescence is brighter than red.
      Longer wavelengths, such as red light, have better penetrability.
      3 PEG functionalization historically reduces non-specific binding and aggregation in buffered media. Carboxylated particles are commonly used for sensing and imaging applications, or to conduct further syntheses in the lab. However, in biological media/buffer (e.g., PBS), they are prone to aggregation, and surface coatings help to reduce this. Moreover, other functionalizations, such as biotin or antibodies, allow for selective targeting.
      4 Threr is information available that it has been used in fluorescent imaging experiments.

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