Developed to meet the needs of the biotechnology industry, this animal component-free medium is designed for single-cell cloning of recombinant CHO cell lines adapted to serum-free suspension culture.
Application
This animal component-free medium is designed to support clonal survival and growth of Chinese Hamster Ovary (CHO) cell lines, with results comparable to the traditional method using 10% fetal bovine serum. Aseptically add 10 mL of 200 mM L-glutamine solution per 500 mL of medium prior to use.
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EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.
A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.
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