14390C

Cellvento^® 4CHO-C Cloning Medium

Animal- component free, chemically defined

• UNSPSC Code: 41106213

Properties

• sterility: sterile; sterile-filtered
• product line: Cellvento^®
• form: liquid
• technique(s): cell culture | mammalian: suitable (suspension)
• components: L-glutamine: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Cellvento^® 4CHO-C Cloning Medium supports CHOZN^® and CHO cell line development and has been developed to replace hydrolysate containing EX-CELL^® CHO Cloning Medium (C6366) at comparable performance. It provides chemically defined media solutions for the entire process from cell line development to production scale.

Chemically definded medium

Application

Cell Culture, Single Cell Cloning

Developed to meet the needs of the biotechnolgy industry, this medium is designed for single cell cloning, stable pool selection and recovery of Chinese Hamster Ovary (CHO) cell lines adapted to suspension culture.

Features and Benefits

Developed to meet the needs of the biotechnology industry, this medium is designed for single-cell cloning of recombinant CHO cell lines adapted to serum-free and hydrolysate free suspension culture.

Packaging

Pack sizes: 14390C-500ML

Components

L-glutamine: no

Caution

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lenthly periods of time.

Reconstitution

Aspectically add 10 mL of 200 mM L-glutamine solution per 500 mL of medium prior to use (if applicable).

Other Notes

The formulation is designed without hydrolysates or components of unknown composition. It contains hypoxanthine and thymidine (HT) but for more selcection flexibility, it does not contain L-glutamine, Methionine sulfoximine (MSC), or Methotrexate (MTX).

Legal Information

6538 is a trademark of American Type Culture Collection

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany

CHOZN is a registered trademark of Merck KGaA, Darmstadt, Germany

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable