X3876

Xylanase from Trichoderma viride

lyophilized powder, ≥100 units/mg protein

• Synonym(s): 1,4-β-D-Xylanxylanohydrolase, endo-1,4-β-Xylanase
• CAS Number: 9025-57-4
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-800-2
• MDL number: MFCD00132594
• EC Number: 3.2.1.8 (BRENDA, IUBMB)
• Specific activity: ≥100 units/mg protein
• Biological source: fungus (Trichoderma viride)

Properties

• biological source: fungus (Trichoderma viride)
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥100 units/mg protein
• mol wt: 22 kDa
• greener alternative product characteristics: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• foreign activity: β-glucosidase and β-xylosidase ≤0.1%, cellulase <0.2%
• greener alternative category: , Enabling
• storage temp.: 2-8°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research

Xylanase is a hemicellulolytic enzyme. It is synthesized by microorganisms like bacteria, yeast and fungi.[1]

Xylanase from Trichoderma viride was shown to have molecular weight of 22 kDa with a Pi of 9.3.

Application

Xylanase from Trichoderma viride has been used as a component of an enzyme mixture for the hydrolysis of hemicellulose-rich solution (autohydrolysate).[2] It has also been used as a cell-wall degrading enzyme to determine the efficiency of p-coumaryl esterase to release p-coumaroyl and feruloyl group.[3]

Biochem/physiol Actions

Xylanase (endo-1,4-β-Xylanase) is involved in the hydrolysis of xylan.[4] It has a wide range of applications in industrial processes such as, bioleeching of craft pulp in paper industry and production of hydrolysates from agro-industrial wastes. Xylanase is also used in nutritional enhancement of lignocellulosic feed and in clarification of juice and wines.[1]

Physical form

Contains sorbitol and sodium acetate buffer salts

Other Notes

One unit will liberate 1 μmole of 4-nitrophenol from 4-nitrophenol-xylan per min at pH 4.5 at 30 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)