Upon creation of a cDNA library, quantitative qPCR or microarray analysis may be performed
WTA1
TransPlex® Whole Transcriptome Amplification Kit
DNA polymerase separate.
Synonym(s):
Transcriptome Amplification Kit
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 reactions | Check Cart for Availability | $3,470.00 |
About This Item
NACRES:
NA.55
UNSPSC Code:
41121800
$3,470.00
Check Cart for Availability
Quality Segment
technique(s)
whole genome amplification: suitable
dilution
(WTA)
input
purified DNA
shipped in
wet ice
storage temp.
−20°C
General description
TransPlex®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.
Application
TransPlex® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA. It has also been used in the amplification of cDNA.[1]
Suitable for use with downstream applications including:
- qPCR
- microarray analysis
- cloning
Biochem/physiol Actions
The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.
Features and Benefits
- Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
- Only 5 ng of starting material required to produce a highly representative library from total RNA
- Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples
Legal Information
TransPlex is a registered trademark of Rubicon Genomics, Inc.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable |
| input purified DNA | input purified RNA | input purified RNA | input purified DNA |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| dilution (WTA) | dilution (WTA) | dilution (WTA) | dilution (WGA) |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Storage Class
12 - Non Combustible Liquids
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Protocols
TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.
Related Content
Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| WTA1-50RXN | 04061835378807 |
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Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used for downstream applications?
1 answer-
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What is the concentration of the dNTPs supplied with the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1?
1 answer-
The dNTP Mix (D6193) is supplied as 10 mM each.
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What is the difference between WTA and WGA? I use the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1.
1 answer-
The starting material for WTA is RNA, whereas for WGA it is genomic DNA. After the RNA is reverse-transcribed into the DNA complement by WTA, the OmniPlex library is formed. This library is in turn amplified by PCR.
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Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used to amplify archival fixed tissue or degraded samples?
1 answer-
Yes, the WTA kit effectively amplifies degraded RNA, including formalin-fixed and paraffin-embedded samples.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, also applicable for ribosomal RNA and/or small RNA amplification?
1 answer-
Using the kit as is, Ribosomal RNA does appear to amplify. However, we have not quantitated how well it is amplified.
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