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V3638

Sigma-Aldrich

Vascular Endothelial Growth Factor 164 from rat

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Synonym(s):

VEGF 164, VEGF-164, VEGF164, Vasculotropin

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About This Item

MDL number:
MFCD01636149
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

rat

Quality Level

200

recombinant

expressed in NSO cells

assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

1.08 ng/mL ED50

mol wt

dimer 50 kDa by SDS-PAGE
monomer 25 kDa by SDS-PAGE

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

UniProt accession no.

P16612

storage temp.

−20°C

Gene Information

rat ... Vegfa(83785)

Related Categories

Application

Vascular Endothelial Growth Factor 164 (VEGF 164) from rat was used for cardiomyocytes culture and Ca2+ transients′ measurement. It was used as a medium M-199 supplement utilized in in vitro incubation for examining the capability of CTS to limit the impact of biologically relevant molecules, in particular epidermal growth factor (EGF), basic fibroblast growth factor (FGF2), and vascular endothelial growth factor A (VEGFA) in rat epididymis segment 1 and 2.

Biochem/physiol Actions

Vascular Endothelial Growth Factor 164 also referred to as VEGF 164 or Vasculotropin is a growth factor that induces endothelial cell growth, vasculogenesis and angiogenesis. VEGF 164 can enhance collagen and fibronectin expression in mesangial cells which in turn stimulates TGF-β1-induced extracellular matrix. It also plays a pivotal role in progressive glomerular fibrosis.
Mitogen for endothelial cells, enhances vascular permeability, activates, and chemoattracts monocytes and is a procoagulant.

Other Notes

164 amino acid variant of rat VEGF.

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% TFA containing 0.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in human umbilical vein endothelial cells.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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The Journal of biological chemistry, 279(32), 33213-33219 (2004-05-15)
Transforming growth factor-beta1 (TGF-beta1) is a potent inducer of extracellular matrix synthesis leading to progressive glomerular fibrosis. The intracellular signaling mechanisms involved in this process remain incompletely understood. The p38 mitogen-activated protein kinase (MAPK) is a major stress signal transducing
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