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T9378

Trypsin inhibitor

crude powder

Synonym(s):

Trypsin Inhibitor from lima beans

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100 mg

Available to ship TODAYfromMILWAUKEE

$211.00
500 mg

Available to ship TODAYfromMILWAUKEE

$882.00
1 g

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$1,260.00

About This Item

CAS Number:
9035-81-8
UNSPSC Code:
12352202
NACRES:
NA.77
EC Number:
232-906-9
MDL number:
MFCD00082102

$211.00

Available to ship TODAYDetails

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Product Name

Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder

biological source

Phaseolus limensis (lima bean)

Quality Segment

200

type

Type II-L

form

crude powder

mol wt

9 kDa

solubility

0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL

storage temp.

2-8°C

General description

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Application

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:
  • in the inhibition of trypsin in human plasma
  • in the termination of proteolytic digestion in rabbit skeletal muscles
  • in the inhibition of salivary glands enzyme extract from Lygus lineolaris
  • in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Biochem/physiol Actions

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

Preparation Note

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Analysis Note

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Other Notes

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.
View more information on Trypsin Inhibitor.

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Trypsin inhibitor from Phaseolus limensis (lima bean) Type II-L, crude powder

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T9378

Trypsin inhibitor from Phaseolus limensis (lima bean)

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Sigma-Aldrich

T2327

Trypsin Inhibitor from Glycine max (soybean)

Trypsin inhibitor from Glycine max (soybean) lyophilized powder, ~10000 U/mg

Sigma-Aldrich

93620

Trypsin inhibitor from Glycine max (soybean)

Trypsin inhibitor from Glycine max (soybean) powder, BioReagent, suitable for cell culture

Sigma-Aldrich

T6522

Trypsin inhibitor from Glycine max (soybean)

biological source

Phaseolus limensis (lima bean)

biological source

Glycine max (soybean)

biological source

Glycine max (soybean)

biological source

Glycine max (soybean)

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

300

form

crude powder

form

lyophilized powder

form

lyophilized powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

solubility

0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL

solubility

-

solubility

0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless

solubility

balanced salt solution: 1 mg/mL, serum-free medium: soluble

mol wt

9 kDa

mol wt

-

mol wt

Mr ~20000

mol wt

20.1 kDa

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000515851
0000515851
0000507134
0000507133
0000507133

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Protocols

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Articles

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Related Content

Global Trade Item Number

SKUGTIN
T9378-500MG04061833265888
T9378-100MG04061835513277
T9378-1G04061826745991

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