T8690

Thioredoxin human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg

• CAS Number: 52500-60-4
• MDL number: MFCD00132423
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli (N-terminal histidine tagged)
• assay: ≥90% (SDS-PAGE)
• form: essentially salt-free, lyophilized powder
• specific activity: ≥5 U/mg
• mol wt: 14 kDa
• technique(s): activity assay: suitable
• solubility: water: soluble 0.50—0.60 mg/mL, clear to slightly hazy, colorless to faint yellow or tan
• suitability: suitable for molecular biology
• UniProt accession no.: P10599
• application(s): cell analysis
• storage temp.: −20°C
• Gene Information: human ... TXN(7295)

Description

General description

Cloned from Jurkat cell cDNA. Identical to the ADF/Trx (GenBank^® Accession Number X77584) and the thioredoxin from human placenta (GenBank^® Accession Number AF085844).

Thioredoxin (TXN) is a 12 kDa ubiquitous protein. The gene is located on human chromosome 9q31.3. TXN is mainly located in the cytoplasm.Thioredoxin contains a redox-active dithiol in the active site. Itexists in several forms, the most prevalent ones being the cytosolic (TRX1) andmitochondrial (TRX2) forms.

Application

Thioredoxin human can be used for in vitro thioredoxin assay. The product can also be used for assessing Thioredoxin reductase (TRXR) enzyme activity.

Thioredoxin human has been used:

• in mitogen-activated protein kinase kinase 4 (MKK4) in vitro kinase assay
• to study its role in transcription regulation, RNA processing and nuclear pore function in human cells
• in proteomics identification of Trx1 reduction target proteins from the hearts of cardiac specific transgenic (Tg)-Trx1 mice

Biochem/physiol Actions

Human Thioredoxin is a recombinant, N-terminal histidine tagged protein. The product induces proliferation in lymphoid cells, fibroblasts as well as in numerous human solid tumor cell lines.The product also stimulates growth in normal as well as leukemic B cell, modifies the transcriptional activity of AP-1and inhibits the apoptosis signal-regulating kinase 1 (ASK1).Thioredoxin (TRX) plays a role in protecting the cells fromelectrophiles, a cytotoxic agent.

Thioredoxin (TXN) has disulfide-reducing activity. It acts as a cytokine to reduce inflammation. TXN functions as a scavenger for reactive oxygen intermediates (ROIs), which are produced in various oxidative stress. Thioredoxin induces cellular proliferation to decrease oxidative stress. TXN is a prognostic biomarker for various cancers, such as lung, pancreatic and hepatocellular carcinoma. It contributes to protein reduction, tissue development and redox regulation.

Mediates the reduction of disulfide bonds in proteins.

Unit Definition

One unit will cause a ΔA₆₅₀ of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Legal Information

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Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)