T7915

Thioredoxin Reductase from Escherichia coli

ammonium sulfate suspension, >25 units/mg protein (Bradford)

• Synonym(s): NADPH:oxidized thioredoxin oxidoreductase
• CAS Number: 9074-14-0
• UNSPSC Code: 12352204
• NACRES: NA.32
• EC Number: 1.8.1.9 (BRENDA, IUBMB)
• MDL number: MFCD00678149
• Specific activity: >25 units/mg protein (Bradford)
• Biological source: Escherichia coli

Properties

• biological source: Escherichia coli
• Quality Segment: 200
• form: ammonium sulfate suspension
• specific activity: >25 units/mg protein (Bradford)
• mol wt: 70 kDa
• technique(s): activity assay: suitable
• UniProt accession no.: P0A9P4
• storage temp.: 2-8°C
• Gene Information: Escherichia coli K12 ... trxB(949054)

Description

General description

Research area: Cell Signaling
Thioredoxin reductases (TrxRs) belongs to family of selenium-containing pyridine nucleotide-disulphide oxidoreductases.

Application

Thioredoxin reductase from Escherichia coli has been used in thioredoxin reductase activity and peroxiredoxin assay.
It has also been used to study the synergy between broccoli sprout extract and selenium in the upregulation of thioredoxin reductase in human hepatocytes.

Thioredoxin Reductase from Escherichia coli can be used in peroxidase-coupled thioredoxin system assay for assessing the peroxidase activitiy of Cys-based thiol peroxidases.Thioredoxin Reductase from Escherichia coli has been used:

• for determining the enzymatic activity of His6-Ahp1p.[1]
• to assay C. elegans TRXR using the TXN-dependent activity assay.
• to investigate the biological reducing systems for organic hydroperoxide resistance R gene (OhrR).
• for enzymatic targeting of auranofin to test its antimicrobial properties.
• in thioredoxin reductase activity and peroxiredoxin assay.
• to study the synergy between broccoli sprout extract and selenium in the upregulation of thioredoxin reductase in human hepatocytes.

Biochem/physiol Actions

An FAD-containing enzyme involved in the transfer of hydrogen from E. coli thioredoxin to other proteins thus providing a powerful disulfide reductase system.

Thioredoxin reductase (TrxR) is an NADPH-dependent oxidoreductase containing one FAD per subunit that reduces the active site disulfide in oxidised thioredoxin (Trx). The molecular weight of the isozymes from mammalian sources vary between 55-67 kDa as compared with 35 kDa in prokaryotes, plants or yeast. The substrate specificity of the mammalian enzyme is much broader than the prokaryotic enzyme reducing both mammalian and E. coli thioredoxins as well as well as non-disulfide substrates such selenite, lipoic acids, lipid hydroperoxides and hydrogen peroxide.

Thioredoxin reductase is a FAD containing enzyme, which transfers the reducing equivalent from NADPH to the disulphide bond of the enzyme by using FAD moiety within the Cys-Ala-Thr-Cys sequence. It can also reduce Trx-S2 to Trx-(SH)2 by using NADPH.[2]

Physical form

Suspension in 3.6 M (NH₄)₂SO₄ containing 30 mM potassium phosphate buffer, pH 7.5, and 2 mM EDTA.

Other Notes

One unit will cause an increase in absorbance of 1.0 at 412 nm (when measured in a coupled assay with E. coli thioredoxin and DTNB) per min per mL at pH 7.0 at 25 °C.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable