SEQR

SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

• Synonym(s): WGA kit, Whole transcriptome amplification
• NACRES: NA.55
• UNSPSC Code: 41121800

Properties

• Quality Segment: 200
• technique(s): whole genome amplification: suitable
• dilution: (WTA)
• input: purified RNA
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The SeqPlex^™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Application

SeqPlex^™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.[1][2]

Features and Benefits

• Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
• Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
• No need to fragment DNA before sequencing.
• Amplifies ds-cDNA in 8 hours or less.
• Compatible with all next generation sequencing platforms except Pacific Bioscience.

Other Notes

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Legal Information

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• wgk: WGK 3