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S7073

SGK1 (60-end), active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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10 μg
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$489.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
99-135 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells

$489.00

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recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

200

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

99-135 nmol/min·mg

mol wt

~73 kDa

UniProt accession no.

O00141

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SGK1(6446)

Biochem/physiol Actions

SGK1 is a member of the serum- and glucocorticoid-induced protein kinase that is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase. SGK1 mRNA is expressed in all tissues and the level of SGK1 mRNA is increased by stimulation with serum or dexamethasone. SGK1 promotes cell survival by phosphorylating and inactivating FKHRL1. SGK and Akt display differences with respect to the efficacy with which they phosphorylate the three regulatory sites on FKHRL1.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

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SGK1 (60-end), active, GST tagged human PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sigma-Aldrich

S7073

SGK1 (60-end), active, GST tagged human

SGK1 (δ1-60, S422D), active human recombinant, expressed in baculovirus infected Sf9 cells (truncated human sequence (a.a. 1-60 deleted, S422D mutation)-histidine-tagged), buffered aqueous solution

Sigma-Aldrich

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SGK1 (δ1-60, S422D), active human

SGK2, active, GST tagged human PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sigma-Aldrich

S6948

SGK2, active, GST tagged human

Sigma-Aldrich

Sigma-Aldrich

C0499

c-KIT (544-end, V654A), active, GST tagged human

Gene Information

human ... SGK1(6446)

Gene Information

human ... SGK1(6446)

Gene Information

human ... SGK2(10110)

Gene Information

human ... KIT(3815)

assay

≥70% (SDS-PAGE)

assay

-

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

specific activity

99-135 nmol/min·mg

specific activity

-

specific activity

70-94 nmol/min·mg

specific activity

6-8 nmol/min·mg

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells (truncated human sequence (a.a. 1-60 deleted, S422D mutation)-histidine-tagged)

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLBW4715
SLBV5167
SLBS1841V

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Related Content

Data Sheet - S7073

Instructions

A Brunet et al.
Molecular and cellular biology, 21(3), 952-965 (2001-01-12)
Serum- and glucocorticoid-inducible kinases (SGKs) form a novel family of serine/threonine kinases that are activated in response to a variety of extracellular stimuli. SGKs are related to Akt (also called PKB), a serine/threonine kinase that plays a crucial role in
T Kobayashi et al.
The Biochemical journal, 344 Pt 1, 189-197 (1999-11-05)
The catalytic domain of serum- and glucocorticoid-induced protein kinase (SGK) is 54% identical with protein kinase B (PKB) and, like PKB, is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol

Global Trade Item Number

SKUGTIN
S7073-10UG04061837756559

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