P5267

L-Proline p-nitroanilide trifluoroacetate salt

≥99% (TLC), suitable for ligand binding assays

• Synonym(s): N-(4-Nitrophenyl)pyrrolidine-2-carboxamide, P-pNA, Pro-pNA
• Empirical Formula (Hill Notation): C₁₁H₁₃N₃O₃ · C₂HF₃O₂
• CAS Number: 108321-19-3
• Molecular Weight: 349.26
• UNSPSC Code: 12352209
• eCl@ss: 32160406
• PubChem Substance ID: 329820223
• NACRES: NA.26
• MDL number: MFCD00069671

Properties

• Product Name: L-Proline p-nitroanilide trifluoroacetate salt, prolyl aminopeptidase substrate
• Quality Segment: 200
• assay: ≥99% (TLC)
• form: powder
• technique(s): ligand binding assay: suitable
• color: white to yellow
• storage temp.: 2-8°C
• SMILES string: OC(=O)C(F)(F)F.[O-][N+](=O)c1ccc(NC(=O)[C@@H]2CCCN2)cc1
• InChI: 1S/C11H13N3O3.C2HF3O2/c15-11(10-2-1-7-12-10)13-8-3-5-9(6-4-8)14(16)17;3-2(4,5)1(6)7/h3-6,10,12H,1-2,7H2,(H,13,15);(H,6,7)/t10-;/m0./s1
• InChI key: KYRVEVYREUUAKH-PPHPATTJSA-N

Description

General description

Proline p-nitroanilide (P-pNA) is a colorimetric substrate for prolyl aminopeptidase (proline iminopeptidase), an enzyme that releases proline from the N-terminus of small peptides.[1]

Application

L-Proline p-nitroanilide trifluoroacetate salt has also been used as a monopeptide substrate for measuring the amidolytic activity of fibrillated peptide catalyst, PC4.[2]

Proline p-nitroanilide (P-pNA) has been used as a substrate for prolyl aminopeptidase (proline iminopeptidase) from cabbage leaves.[3]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)