No specific timing is recommended for heating and cooling. A gentle warming to 50 °C for a few minutes, followed by cooling to room temperature or as required in the experiment, is suggested.
P1530
Polyinosinic–polycytidylic acid sodium salt
10% Poly (I:C) with buffer salts, TLR ligand tested
Synonym(s):
Poly (I:C), Poly(I) • Poly(C)
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 mg | Available to ship TODAYfromMILWAUKEE | $231.00 | |
| 100 mg | Available to ship TODAYfromMILWAUKEE | $690.00 |
About This Item
Assay:
(Free nucleotides NMT 1% (TLC))
Form:
lyophilized powder
Solubility:
H2O: 10 mg/mL
Storage temp.:
−20°C
Quality Segment
assay
(Free nucleotides NMT 1% (TLC))
form
lyophilized powder
storage condition
desiccated
solubility
H2O: 10 mg/mL
storage temp.
−20°C
SMILES string
[Na].[P](=O)(OC[C@H]3O[C@H]([C@@H]([C@@H]3O)O)[n]4c5ncnc(c5nc4)O)(O)O.[P](=O)(OC[C@H]1O[C@H]([C@@H]([C@@H]1O)O)N2C=CC(=NC2=O)N)(O)O
InChI
1S/C10H13N4O8P.C9H14N3O8P.Na/c15-6-4(1-21-23(18,19)20)22-10(7(6)16)14-3-13-5-8(14)11-2-12-9(5)17;10-5-1-2-12(9(15)11-5)8-7(14)6(13)4(20-8)3-19-21(16,17)18;/h2-4,6-7,10,15-16H,1H2,(H,11,12,17)(H2,18,19,20);1-2,4,6-8,13-14H,3H2,(H2,10,11,15)(H2,16,17,18);/t
InChI key
GETQKWBYQAUFQM-VRQAYDGLSA-N
Application
TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.
Biochem/physiol Actions
Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.
Preparation Note
The product requires ionic strength to maintain the double-strand structure. To prevent denaturation, reconstitute in solutions with physiological salt concentrations (e.g., saline solution). Reconstitution may require heating (50 °C) and cooling to achieve re-annealing.
Other Notes
Double-stranded homopolymer.
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| assay ≥99% (less than 1% free nucleotides, TLC) | assay ≥99% (less than 1% free nucleotides, TLC) | assay ≥99% (less than 1% free nucleotides, TLC) | assay ≥99% (TLC) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility H2O: 10 mg/mL | solubility H2O: 10 mg/mL | solubility - | solubility - |
| storage condition desiccated | storage condition desiccated | storage condition desiccated | storage condition - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P1530-25MG | 04061834355663 |
| P1530-100MG | 04061834355649 |
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P1530 [Reconstitution may prove difficult, and require heating (50 °C) and cooling to achieve re-annealing] How long heating and cooling?
1 answer-
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Is this product tissue culture sterile?
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Product P1530 is not tissue culture sterile
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What is the molecular weight of Polyinosinic-Polycytidylic acid Sodium Salt (P1530)?
1 answer-
The Polyinosinic-Polycytidylic acid Sodium Salt (P1530) is a double-stranded homopolymer with variable molecular weights. The variable range has been observed at 400-1250 base pairs; however, this may vary from lot to lot.
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What is the length of the poly(I).(C)?
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The product is a double-stranded homopolymer without a specified length. Historical data have shown a broad lot-to-lot variability with an approximate range of 200 - >1000 bp length.
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What is the length of the Poly/I).poly(C)?
1 answer-
The product is double-stranded homopolymer without specified length,. Historical data have shown broad lot to lot variability with an approximately range of 200 - >1000 bp in length.
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Could you please elucidate about the purity of the compound and if its sutible for human use or GMP grade?
1 answer-
The minimum purity specification for this item is 99%. However, this product it is not suitable for human use or GMP applications. Unless otherwise stated this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Please see the link to below to review addtional information regarding our various quality levels:
https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/m-clarity-programHelpful?
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