Product P0065 (Phospholipase D) is sold by activity units per milliliter (units/mL). The protein amount is not measured; only the units/mL are provided for this product.
P0065
Phospholipase D from Streptomyces chromofuscus
≥50,000 units/mL, buffered aqueous glycerol solution
Synonym(s):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25000 units | Check Cart for Availability | $284.00 |
About This Item
CAS Number:
UNSPSC Code:
41106300
NACRES:
NA.77
EC Number:
232-639-8
MDL number:
Specific activity:
≥50,000 units/mL
form
buffered aqueous glycerol solution
Quality Segment
specific activity
≥50,000 units/mL
mol wt
~60 kDa
shipped in
dry ice
storage temp.
−70°C
General description
Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.
Application
Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).
Biochem/physiol Actions
Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline. PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.
Physical form
Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).
Other Notes
One unit will release 1 μmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 30 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥50,000 units/mL | specific activity ≥150 units/mg solid | specific activity ≥50 units/mg dry wt. | specific activity ≥60 units/mg protein |
| form buffered aqueous glycerol solution | form lyophilized powder | form lyophilized solid | form lyophilized powder |
| shipped in dry ice | shipped in - | shipped in - | shipped in - |
| storage temp. −70°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~60 kDa | mol wt - | mol wt - | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| P0065-25KU | 04061834262077 |
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