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N8010

4-Nitrophenyl 4-guanidinobenzoate hydrochloride

protease substrate, ≥95.0% (TLC), powder

Synonym(s):

4-Guanidinobenzoic acid 4-nitrophenylester hydrochloride, NPGB, pNPGB

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500 mg
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$334.00
1 g

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About This Item

Empirical Formula (Hill Notation):
C14H12N4O4 · HCl
CAS Number:
19135-17-2
Molecular Weight:
336.73
NACRES:
NA.32
PubChem Substance ID:
24897847
UNSPSC Code:
12352204
EC Number:
242-831-3
MDL number:
MFCD00037010
Beilstein/REAXYS Number:
8024554

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Product Name

4-Nitrophenyl 4-guanidinobenzoate hydrochloride, protease inhibitor and substrate

Quality Segment

200

assay

≥95.0% (TLC)

form

powder

solubility

formic acid: soluble 49.00-51.00 mg/mL

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)Nc1ccc(cc1)C(=O)Oc2ccc(cc2)[N+]([O-])=O

InChI

1S/C14H12N4O4.ClH/c15-14(16)17-10-3-1-9(2-4-10)13(19)22-12-7-5-11(6-8-12)18(20)21;/h1-8H,(H4,15,16,17);1H

InChI key

PKSBDZOBYIKNGY-UHFFFAOYSA-N

General description

4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) is a trypsin substrate. It is more water-soluble than 4-methylumbelliferyl 4-guanidinobenzoate and is preferred in spectrophotometric assay.[1]

Application

4-Nitrophenyl 4-guanidinobenzoate hydrochloride has been used:
  • as a substrate for trypsin for active site titration experiments
  • for pre-treating of mosquito eggs in the interplasmid transposition assay [2]
  • as a component of isotonic buffer to moisten filter paper for mosquito embryo collection[3]

Biochem/physiol Actions

4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) inhibits phenoloxidase enzyme and delays embryo chorionic membrane hardening.[3]

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4-Nitrophenyl 4-guanidinobenzoate hydrochloride protease inhibitor and substrate

Sigma-Aldrich

N8010

4-Nitrophenyl 4-guanidinobenzoate hydrochloride

4-Nitrophenyl β-D-glucuronide ≥98% (TLC)

Sigma-Aldrich

N1627

4-Nitrophenyl β-D-glucuronide

4-Nitrophenyl β-D-glucuronide ≥99.0% (TLC)

Sigma-Aldrich

73677

4-Nitrophenyl β-D-glucuronide

4-Nitrophenyl α-D-mannopyranoside α-mannosidase substrate

Sigma-Aldrich

N2127

4-Nitrophenyl α-D-mannopyranoside

assay

≥95.0% (TLC)

assay

≥98% (TLC)

assay

≥99.0% (TLC)

assay

≥98% (TLC)

solubility

formic acid: soluble 49.00-51.00 mg/mL

solubility

H2O: soluble 100 mg/mL, clear

solubility

H2O: 0.1 g/mL, clear, faintly yellow

solubility

DMF: 50 mg/mL

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

form

powder

form

powder

form

powder or crystals

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000427384
0000427384
0000361401
0000322469
0000322468

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Global Trade Item Number

SKUGTIN
N8010-1G04061834118336
N8010-500MG04061834118343

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