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N2250

Naphthol AS-BI phosphate disodium salt hydrate

fluorogenic, ≥96.0% (HPLC), powder

Synonym(s):

2-Naphthalenecarboxamide, 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)-, disodium salt hydrate

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100 mg
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$80.10
50 mg
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$82.20
500 mg
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$234.00
1 g
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$397.00
5 g
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$1,500.00

About This Item

Empirical Formula (Hill Notation):
C18H13BrNNa2O6P · xH2O
CAS Number:
207569-04-8
Molecular Weight:
496.16 (anhydrous basis)
UNSPSC Code:
12352204
PubChem Substance ID:
24897558
NACRES:
NA.83

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Product Name

Naphthol AS-BI phosphate disodium salt hydrate, ≥96.0% (HPLC)

Quality Segment

200

assay

≥96.0% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless to yellow

storage temp.

−20°C

SMILES string

O.[Na+].[Na+].COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP([O-])([O-])=O

InChI

1S/C18H15BrNO6P.2Na.H2O/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24;;;/h2-10H,1H3,(H,20,21)(H2,22,23,24);;;1H2/q;2*+1;/p-2

InChI key

WNPKMQCBZKBNOS-UHFFFAOYSA-L

General description

Substrate for the histochemical demonstration of acid and alkaline phosphatase.
Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) is a substituted naphthol.

Application

Naphthol AS-BI phosphate disodium salt hydrate is suitable to use:
  • in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
  • as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
  • to perform alkaline phosphatase reaction

Biochem/physiol Actions

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

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Naphthol AS-BI phosphate disodium salt hydrate ≥96.0% (HPLC)

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Sigma-Aldrich

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Sigma-Aldrich

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assay

≥96.0% (HPLC)

assay

≥99% (HPLC)

assay

≥98% (HPLC)

assay

≥97% (TLC)

solubility

water: 50 mg/mL, clear, colorless to yellow

solubility

water: 50 mg/mL, clear, colorless to faintly yellow

solubility

methanol: 50 mg/mL

solubility

DMF: 50 mg/mL

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN6231
BCCM7980
BCCH6248
BCCD0900
BCBL3289V

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Global Trade Item Number

SKUGTIN
N2250-5G04061834110286
N2250-1G04061834110279
N2250-100MG04061833273098
N2250-50MG04061833273111
N2250-500MG04061833273104

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