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M7292

M16 Medium

With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

Synonym(s):

Cell Culture Medium, Cell Culture Medium M16, M16 Medium Solution

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50 mL
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$41.60
100 mL
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$74.00

About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

$41.60

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Quality Segment

300

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

components

NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

Application

M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

Preparation Note

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Analysis Note

Presence of particulates in solution will not affect product′s performance.
This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

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This Item
M7167R515814610C
M16 Medium With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

Sigma-Aldrich

M7292

M16 Medium

M2 medium With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

Sigma-Aldrich

M7167

M2 medium

RPMI-1640 Medium HEPES Modification, With 25 mM HEPES and L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R5158

RPMI-1640 Medium

SAFC

SAFC

14610C

EX-CELL® 610-HSF Serum-Free Medium for Hybridoma Cells

technique(s)

cell culture | embryo: suitable

technique(s)

cell culture | embryo: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

components

NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)

components

HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
NaHCO3: 2 g/L
HEPES: 5.96 g/L
glucose: 2 g/L (Dextro)

components

glucose: 3.45 g/L
NaHCO3: 1.60 g/L
L-glutamine: 0.333 g/L
HEPES: 7.5 mM

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000398219
0000398220
0000398220
0000398219
0000381902

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Articles

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Qian-Qian Sha et al.
Nature communications, 9(1), 3477-3477 (2018-08-30)
Trimethylation of histone H3 on lysine-4 (H3K4me3) is associated with gene-regulatory elements, but its transcription-independent function in cell division is unclear. CxxC-finger protein-1 (CFP1) is a major mediator of H3K4 trimethylation in mouse oocytes. Here we report that oocyte-specific knockout
Marco Ragusa et al.
RNA biology, 16(9), 1237-1248 (2019-05-29)
Circular RNAs (circRNAs) have a critical role in the control of gene expression. Their function in spermatozoa (SPZ) is unknown to date. Twenty-eight genes, involved in SPZ/testicular and epididymal physiology, were given in circBase database to find which of them
Hong-Bin Liu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 6(15), 1900295-1900295 (2019-08-14)
A number of genes involved in zygotic genome activation (ZGA) have been identified, but the RNA-binding maternal factors that are directly related to ZGA in mice remain unclear. The present study shows that maternal deletion of Igf  2bp2 (also commonly known
View All Related Papers

Global Trade Item Number

SKUGTIN
M7292-100ML04061834061854
M7292-50ML04061834061878

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