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M7256

Sigma-Aldrich

N,N′-Methylenebisacrylamide

suitable for electrophoresis (after filtration or allowing insolubles to settle)

Synonym(s):

BIS

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About This Item

Linear Formula:
(CH2=CHCONH)2CH2
CAS Number:
110-26-9
Molecular Weight:
154.17
Beilstein/REAXYS Number:
1706297
EC Number:
203-750-9
MDL number:
MFCD00008625
UNSPSC Code:
41105319
PubChem Substance ID:
24897148
NACRES:
NB.22

form

powder

Quality Level

200

technique(s)

electrophoresis: suitable

suitability

suitable for electrophoresis (after filtration or allowing insolubles to settle)

SMILES string

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

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Application

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

Other Notes

Cross-linking agent, for the preparation of polyacrylamide (from 01698 Acrylamide puriss.) used as gel in electrophoresis for the separation, analysis and characterization of nucleic acids, proteins and other charged species; A.H. Gordon, Electrophoresis of Proteins in Polyacrylamide and Starch Gels, Vol. 1, Part. I, of "Laboratory Techniques in Biochemistry and Mol. Biology".

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Carc. 1B - Muta. 1B - Repr. 2 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

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D. Rickwood, B.D. Hames
Gel Electrophoresis of Nucleic Acids: A Practical Approach (1983)
A Bringard et al.
Electrophoresis, 11(10), 802-809 (1990-10-01)
The principle and some applications of multiphasic electrophoresis with diversified spacers are described. The method combines previously published concepts of introducing highly diversified spacers into the stack of an isotachophoretic system with the theory of snow- and telescope-electrophoresis. The link
A J Aho et al.
British journal of anaesthesia, 115(2), 258-266 (2015-07-04)
Concomitantly recorded Bispectral Index® (BIS) and Entropy™ values sometimes show discordant trends during general anaesthesia. Previously, no attempt had been made to discover which EEG characteristics cause discrepancies between BIS and Entropy. We compared BIS and Entropy values, and analysed
Jean Guglielminotti et al.
Anesthesiology, 122(5), 985-993 (2015-03-03)
Individual assessment of the amplitude of a physiologic reflex evoked by a standardized noxious test (SNT) before surgical stimulation has been suggested to predict movement upon the forthcoming surgical stimulation. This study aimed to compare the ability of pupillary dilatation
Han Gao et al.
Macromolecular rapid communications, 34(1), 63-68 (2012-10-20)
Traditional pH-sensitive hydrogels inevitably suffer strength deterioration while the responsive weak acid or base groups are in the ionized state. In this study, we report on a facile approach to fabricate a novel pH-sensitive high-strength hydrogel from copolymerization of two
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