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M5755

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

Synonym(s):

MESA Buffer

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1 L

Available to ship TODAYfromMILWAUKEE

$315.00

About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

$315.00


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grade

Molecular Biology

Quality Segment

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H2O: soluble, faintly yellow

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

room temp

General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Preparation Note

1x MESA buffer contains:
  • 40 mM MOPS
  • 10 mM sodium acetate
  • 1 mM EDTA (pH 8.3)
Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

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This Item
T8280T7527PPB008
technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

-

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

pHast Pack

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

form

powder

form

powder blend

form

powder blend

form

powder

pH

6.8-7.2(reconstituted per directions)

pH

8.1-8.5

pH

8.1-8.5 (5 ×)

pH

8.3


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Related Content

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.






Global Trade Item Number

SKUGTIN
M5755-1L04061834060314

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