L1378

L-Lactic Dehydrogenase from bovine muscle

Type X, ammonium sulfate suspension, ≥600 units/mg protein

• Synonym(s): (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD, Lactate
• CAS Number: 9001-60-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-617-8
• MDL number: MFCD00131466
• EC Number: 1.1.1.27 (BRENDA, IUBMB)
• Specific activity: ≥600 units/mg protein
• Assay: 3.0—25.0 mg protein/mL (biuret)
• Biological source: bovine muscle

Properties

• biological source: bovine muscle
• Quality Segment: 200
• type: Type X
• assay: 3.0—25.0 mg protein/mL (biuret)
• form: ammonium sulfate suspension
• specific activity: ≥600 units/mg protein
• manufacturer/tradename: Sigma-Aldrich
• technique(s): activity assay: suitable
• color: white
• pH: 7.5
• UniProt accession no.: P19858, Q5E9B1
• application(s): life science and biopharma
• foreign activity: pyruvate kinase ≤0.01%
• storage temp.: 2-8°C
• Gene Information: cow ... LDHA(281274), LDHB(281275)

Description

General description

Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.

Application

L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.

Physical form

Crystalline suspension in 2.4 M (NH₄)₂SO₄ solution, pH 6.0

Analysis Note

Protein determined by biuret.

Other Notes

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 10 - Combustible liquids
• ppe: Eyeshields, Gloves, type N95 (US)