G3907

Glutathione−Agarose

set of 3 pre-packed columns (2.5 ml each), (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

• Synonym(s): GSH-agarose, S-linked glutathione agarose
• UNSPSC Code: 41106500
• NACRES: NA.56
• MDL number: MFCD00131200
• Matrix: cross-linked 4% beaded agarose

Properties

• Quality Segment: 200
• form: (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)
• analyte chemical class(es): proteins (GST)
• packaging: set of 3 pre-packed columns (2.5 ml each)
• technique(s): immunoprecipitation (IP): suitable, protein purification: suitable
• matrix: cross-linked 4% beaded agarose
• storage temp.: 2-8°C

Description

General description

The resin in these columns consists of glutathione attached through the sulfur to epoxy activated, 4% cross-linked beaded agarose resulting in a 12 atom spacer. [1]

Application

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of glutathione binding enzymes such as glutathione-S-transferase, glutathione peroxidase, and glyoxalase I.[2]
The product was used in the design, synthesis and biological evaluation of new tryptamine and tetrahydro-β-carboline-based selective inhibitors of CDK4.[3] It was also used to study Fascaplysin-inspired diindolyls as selective inhibitors of CDK4/cyclin D1.[4]

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

Physical form

1:1 suspension in a 0.5 M NaCl + 20% ethanol solutionl

Other Notes

Suspension of Product No. G 4510

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: 180.1 °F
• flash_point_c: 82.3 °C