G0799

β-Glucuronidase from Escherichia coli

>20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution

• Synonym(s): β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-606-8
• MDL number: MFCD00199013
• EC Number: 3.2.1.31 (BRENDA, IUBMB)
• Specific activity: >20,000,000 units/g protein
• Recombinant: expressed in E. coli
• Concentration: >1 mg/mL

Properties

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: aqueous glycerol solution
• specific activity: >20,000,000 units/g protein
• mol wt: 74 kDa
• concentration: >1 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

β-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.[1]

Application

β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.[2]

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Effective in the hydrolysis of steroid glucuronides.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Physical form

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Highly purified solution in 50% glycerol

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable