F1804

ANTI-FLAG^® M2 antibody, Mouse monoclonal

1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

• Synonym(s): flag antibody, Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG^® M2
• NACRES: NA.32
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: M2, monoclonal
• Application: —
• Citations: 9676

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: M2, monoclonal
• form: buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
• purified by: affinity chromatography (affinity tag purification)
• species reactivity: all
• concentration: 1.0-1.2 mg/mL
• isotype: IgG1
• immunogen sequence: DYKDDDDK
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) [1], immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Learn more product details in our FLAG^® application portal.

Biochem/physiol Actions

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Storage Class: 10 - Combustible liquids