C7988

Anti-Cre antibody, Mouse monoclonal

clone 7-23, purified from hybridoma cell culture

• Synonym(s): Mouse Anti-Recombinase cre
• UNSPSC Code: 12352203
• NACRES: NA.56
• MDL number: MFCD02262736
• Conjugate: unconjugated
• Clone: 7-23, monoclonal
• Application: ELISA (i), FACS, ICC, IHC, IP, WB
• Citations: 12

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 7-23, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~38 kDa
• concentration: ~2 mg/mL
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 0.5-1 μg/mL using recombinant Cre recombinase
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Cre (mouse IgG1 isotype) is derived from the 7-23 hybridoma produced by the fusion of mouse myeloma cells (X63-Ag8.653) and splenocytes from BALB/c mice immunized with Cre protein. Cre recombinase is a member of the integrase family of proteins that are site-specific recombinases.The protein is expressed in P1 bacteriophage.

Application

Anti-Cre antibody ,Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunohistochemistry
• immunocytochemistry
• flow cytometry
• immunostaining

Biochem/physiol Actions

Cre recombinase enables DNA recombination between two recognition sites called loxP. Each one of these sites (34 bp long) contain two 13 bp palindromic flanking sequences and in between them is a core spacer sequence 8 bp long. The recombination event takes place within the spacer area of the two loxP sites. Cre/loxP system manipulates genes in mammalian systems by making deletions, gene replacements, insertions (knockin), conditional gene targeting (knockout), and point mutations. Monoclonal antibodies to the Cre protein are an important tool for the identification of Cre recombinase in different mammalian systems.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution should be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable